Yr Athro Diane Kelly

Athro Emeritws (Meddygaeth)

Medical School

+44 (0) 1792 602184

d.kelly@abertawe.ac.uk

Dolenni Ymchwil

ResearchGate

Trosolwg

Dechreuodd Diane ymddiddori mewn "Bio-drawsnewidiadau a Cytochromes P450" fel myfyriwr israddedig a datblygwyd hyn ymhellach yn ystod ei PhD, prosiect ar y cyd rhwng Cronfa Ymchwil Canser Imperial, (CRUK bellach) a Phrifysgol Abertawe (UM). Dechreuodd ei gyrfa ôl-ddoethurol ym Mhrifysgol Sheffield, yn gyntaf yn yr Adran Geneteg ac yn ddiweddarach yn Sefydliad Biotechnoleg Wolfson, lle mae hi wedi dilyn ffocws ymchwil ar cytocrom moctobial P450 ers hynny.

Ar ôl symud o Sheffield i Brifysgol Aberystwyth, daeth Diane nol i Brifysgol Abertawe fel Darllenydd ac yna Athro yn Ysgol Glinigol Abertawe a ffurfiwyd yn ddiweddar, yn 2004. Mae ei hymchwil yn parhau i fod ar Cytocrom Microbial P450 sy'n gysylltiedig â'u bioamrywiaeth, gan gynnwys mycobacterial P450's ac ystod o ffyngau, gyda phwyslais ar nodi targedau ar gyfer asiantau gwrthficrobaidd mewn meddygaeth ac amaethyddiaeth a'r cynnydd cysylltiedig mewn ymwrthedd i therapïau cyfredol. Mae Diane wedi cyhoeddi mwy na 200 o bapurau cysylltiedig a adolygwyd gan gymheiriaid.

Mae Diane yn Gyd-arweinydd SU ar brosiect a ariennir gan Gronfa Datblygu Rhanbarthol Ewrop (ERDF), BEACON. Mae hwn yn brosiect gwerth £ 18.3 miliwn gyda chefnogaeth yr UE a Llywodraeth Cymru. Enillodd BEACON wobr RegioStars 2014 am y Prosiect Rhanbarthol gorau yn yr UE ac mae'n dathlu 10 mlynedd (2020) o gydweithrediadau llwyddiannus rhwng HEI Cymru a Diwydiant.

Mae Diane yn adolygydd panel ar gyfer grantiau UKRC ac ar gyfer Sefydliad Ymchwil Wyddonol yr Iseldiroedd (NWO), mae Vidi yn rhoi grantiau yng Nghynllun Cymhellion Ymchwil Arloesol y Parth Gwyddoniaeth.

Mae Diane yn aelod o Bwyllgor Enwebiadau'r Brifysgol.

Trwy gydol ei gyrfa mae Diane wedi teimlo'n angerddol dros gydraddoldeb o fewn a tu allan o'r gweithle ac mae wedi bod yn rhan o waith siarter SU Athena SWAN er 2008, yn gyntaf fel aelod o ddeigryn hunanasesu SU (SAT) yn ennill y wobr Efydd yn 2009 ac yna fel cadeirydd ar gyfer adnewyddiad llwyddiannus y Wobr Efydd yn 2012. Mae wedi parhau fel aelod o SU SAT a enillodd wobr Sefydliadol Arian yn 2017. Mae Diane yn cadeirio pwyllgor EDI a SAT yr Ysgol Feddygaeth, dyfarnwyd Efydd i'r Ysgol yn 2014, Arian yn 2016 ac Arian 2019. Mae hi'n aelod sefydlu o grŵp Strategaeth SWAN Prifysgol Athena ac yn cadeirio ac yn eistedd ar baneli asesu SWAN Athena y DU.

Meysydd Arbenigedd

Cytochromau microbaidd P450 (CYP’s)
Partneriaid Redox (o enynnau i broteinau a bioamrywiaeth).
Bioleg sterol ffwngaidd
Gwrthiant gwrthficrobaidd

Uchafbwyntiau Gyrfa

Ymchwil

Dangoswyd bod baich dynol afiechyd ffwngaidd yn fwy na chanser y fron, malaria neu TB. Mewn amaethyddiaeth mae clefyd ffwngaidd yn fater o bwys ar gyfer Cyfrinachedd Bwyd. Y farchnad fyd-eang yw $ 13.7bn ar gyfer cyffuriau a $ 7bn ar gyfer ffwngladdiadau pa.

Pwyslais ymchwil mawr y mae Diane yn rhan ohono (http://www.p450swansea.co.uk/) fu astudio swyddogaeth, strwythur a gwaharddiad cytocromau P450 (CYP) yn benodol CYP51, y targed ar gyfer imidazole a triazole gwrth- ffyngau. Mae CYP51 yn ensym mewn biosynthesis sterol (14a-demethylation lanosterol) sy'n arwain yn agos at ergosterol mewn pilenni ffwngaidd, neu golesterol mewn pilenni celloedd dynol, yn y drefn honno. Mae angen datblygu cyffuriau newydd sy'n ddetholus, heb ryngweithiadau niweidiol rhwng cyffuriau / cyffuriau (gan fod metaboledd mwyaf cyffuriau gan CYPau eraill) a brwydro yn erbyn y broblem gwrthsefyll cyffuriau mewn ffyngau. Yn aml, mae'r cleifion dan fygythiad imiwn ac ar driniaeth hir neu broffylatig.

Mae ymchwil yn Abertawe wedi arwain at ddatblygu deunyddiau a dulliau sy'n cynnwys ynysu a nodweddu proteinau CYP newydd o bathogenau ffwngaidd meddygol a phlanhigion, gyda phwyslais ar nodi dull gweithredu a mecanweithiau gwrthiant.

Prif Wobrau Cydweithrediadau

Cyhoeddiadau

Uchafbwyntiau Ymchwil

Warrilow, A., Nishimoto, A., Parker, J., Price, C., Flowers, S., Kelly, D., Rogers, P., & Kelly, S. (2019). The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions. Antimicrobial Agents and Chemotherapy, 63(5)
https://doi.org/10.1128/aac.02586-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48946
Lamb, D., Kelly, D., Venkateswarlu, K., Manning, N., Bligh, H., Schunck, W., Kelly, S., & Kanamarlapudi, V. (1999). Generation of a Complete, Soluble, and Catalytically Active Sterol 14α-Demethylase−Reductase Complex†. Biochemistry, 38(27), 8733-8738.
https://doi.org/10.1021/bi9825089, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25106
Venkateswarlu, K., Kelly, D., Manning, N., Kelly, S., & Kanamarlapudi, V. (1998). NADPH cytochrome P-450 oxidoreductase and susceptibility to ketoconazole. Antimicrobial Agents and Chemotherapy, 42
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25111
Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (1997). Characterization of Saccharomyces cerevisiae CYP51 and a CYP51 fusion protein with NADPH cytochrome P-450 oxidoreductase expressed in Escherichia coli. Antimicrobial Agents and Chemotherapy, 41
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25119
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment, 656, 531-539.
https://doi.org/10.1016/j.scitotenv.2018.11.393, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46109
Salvage, R., Hull, C., Kelly, D., & Kelly, S. (2014). Use of 70% alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms. Future Microbiology, 9(10), 1123-1130.
https://doi.org/10.2217/fmb.14.73, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19624
Venkateswarlu, K., Lamb, D., Kelly, D., Manning, N., Kelly, S., & Kanamarlapudi, V. (1998). The N-Terminal Membrane Domain of Yeast NADPH-Cytochrome P450 (CYP) Oxidoreductase Is Not Required for Catalytic Activity in Sterol Biosynthesis or in Reconstitution of CYP Activity. Journal of Biological Chemistry, 273(8), 4492-4496.
https://doi.org/10.1074/jbc.273.8.4492, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25109
Fang, G., Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (1994). STUDIES ON YEAST HOST STRAINS FOR HETEROLOGOUS P450 EXPRESSION. Biotechnology Letters, 16
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25127
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Warrilow, A., Ugochukwu, C., Lamb, D., Kelly, D., & Kelly, S. (2008). Expression and Characterization of CYP51, the Ancient Sterol 14alpha-demethylase Activity for Cytochromes P450 (CYP), in the White-Rot Fungus Phanerochaete chrysosporium. Lipids, 43(12), 1143-1153.
https://doi.org/10.1007/s11745-008-3239-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6350
Barber, R., Hickenbotham, P., Hatch, T., Kelly, D., Topchiy, N., Almeida, G., Jones, G., Johnson, G., Parry, J., Rothkamm, K., Dubrova, Y., & Johnson, G. (2006). Radiation-induced transgenerational alterations in genome stability and DNA damage. Oncogene, 25(56), 7336-7342.
https://doi.org/10.1038/sj.onc.1209723, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa17857
Lamb, D., Kaderbhai, N., Venkateswarlu, K., Kelly, D., Kelly, S., Kaderbhai, M., & Kanamarlapudi, V. (2001). Human Sterol 14α-Demethylase Activity Is Enhanced by the Membrane-Bound State of Cytochrome b5. Archives of Biochemistry and Biophysics, 395(1), 78-84.
https://doi.org/10.1006/abbi.2001.2566, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25098
Lamb, D., Warrilow, A., Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (2001). Activities and Kinetic Mechanisms of Native and Soluble NADPH–Cytochrome P450 Reductase. Biochemical and Biophysical Research Communications, 286(1), 48-54.
https://doi.org/10.1006/bbrc.2001.5338, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25100
Zhou, W., Warrilow, A., Thomas, C., Ramos, E., Parker, J., Price, C., Vanderloop, B., Fisher, P., Loftis, M., Kelly, D., Kelly, S., & Nes, W. (2018). Functional importance for developmental regulation of sterol biosynthesis in Acanthamoeba castellanii. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1863(10), 1164-1178.
https://doi.org/10.1016/j.bbalip.2018.07.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa41148
Plácido, J., López, S., Meissner, K., Kelly, D., Kelly, S., & Placido Escobar, J. (2019). Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment, 688, 751-761.
https://doi.org/10.1016/j.scitotenv.2019.06.342, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50904
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment, 689, 10-20.
https://doi.org/10.1016/j.scitotenv.2019.06.390, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50905
Plácido, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology, 196, 106163
https://doi.org/10.1016/j.fuproc.2019.106163, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51273
Warrilow, A., Jackson, C., Parker, J., Marczylo, T., Kelly, D., Lamb, D., & Kelly, S. (2008). Identification, Characterization, and Azole-Binding Properties of Mycobacterium smegmatis CYP164A2, a Homolog of ML2088, the Sole Cytochrome P450 Gene of Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 53(3), 1157-1164.
https://doi.org/10.1128/AAC.01237-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6351
Parker, J., Warrilow, A., Price, C., Mullins, J., Kelly, D., & Kelly, S. (2014). Resistance to antifungals that target CYP51. Journal of Chemical Biology, 7(4), 143-161.
https://doi.org/10.1007/s12154-014-0121-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18694
Colley, T., Sehra, G., Chowdhary, A., Alanio, A., Kelly, S., Kizawa, Y., Armstrong-James, D., Fisher, M., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Nishimoto, Y., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J...., & Rapeport, G. (2018). In Vitro and In Vivo Efficacy of a Novel and Long-Acting Fungicidal Azole, PC1244, on Aspergillus fumigatus Infection. Antimicrobial Agents and Chemotherapy, 62(5), AAC.01941-17
https://doi.org/10.1128/aac.01941-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa38876
http://dx.doi.org/10.1128/aac.01941-17
Martel, C., Parker, J., Warrilow, A., Rolley, N., Kelly, S., Kelly, D., Parker, J., Hull, C., & Warrilow, A. (2010). Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14alpha-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B. Antimicrobial Agents and Chemotherapy, 54(11), 4920-4923.
https://doi.org/10.1128/AAC.00349-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6356
Jackson, C., Lamb, D., Marczylo, T., Parker, J., Manning, N., Kelly, D., & Kelly, S. (2003). Conservation and cloning of CYP51: a sterol 14α-demethylase from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications, 301(2), 558
https://doi.org/10.1016/S0006-291X(02)03078-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6859
Warrilow, A., Mullins, J., Hull, C., Parker, J., Lamb, D., Kelly, D., Kelly, S., & Mullins, J. (2012). S279 Point Mutations in Candida albicans Sterol 14- Demethylase (CYP51) Reduce In Vitro Inhibition by Fluconazole. Antimicrobial Agents and Chemotherapy, 56(4), 2099-2107.
https://doi.org/10.1128/AAC.05389-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14242
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37567
Warrilow, A., Parker, J., Price, C., Rolley, N., Nes, W., Kelly, D., & Kelly, S. (2019). Isavuconazole and voriconazole inhibition of sterol 14α-demethylases (CYP51) from Aspergillus fumigatus and Homo sapiens. International Journal of Antimicrobial Agents
https://doi.org/10.1016/j.ijantimicag.2019.07.011, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51096
Warrilow, A., Parker, J., Price, C., Garvey, E., Hoekstra, W., Schotzinger, R., Wiederhold, N., Nes, W., Kelly, D., & Kelly, S. (2017). The Tetrazole VT-1161 Is a Potent Inhibitor of Trichophyton rubrum through Its Inhibition of T. rubrum CYP51. Antimicrobial Agents and Chemotherapy, 61(7), e00333-17
https://doi.org/10.1128/AAC.00333-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34176
Melo, N., Moran, G., Warrilow, A., Dudley, E., Smith, S., Sullivan, D., Lamb, D., Kelly, D., Coleman, D., & Kelly, S. (2008). CYP56 (Dit2p) in Candida albicans: Characterization and Investigation of Its Role in Growth and Antifungal Drug Susceptibility. Antimicrobial Agents and Chemotherapy, 52(10), 3718-3724.
https://doi.org/10.1128/AAC.00446-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6349
Bader, O., Kuhns, M., Hull, C., Parker, J., Bader, M., Tintelnot, K., Seiboldt, M., Kelly, D., Kelly, S., Gross, U., & Weig, M. (2009). Frequency of azole resistance phenotypes in the two most prevalent human pathogenic yeast, Candida albicans and Candida glabrata. MYCOSES, 52(5)-421.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa16023
Colley, T., Alanio, A., Kelly, S., Sehra, G., Kizawa, Y., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Anderson-Dring, L., Nakaoki, T., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J., Cooke, M., Bretagne, S...., & Rapeport, G. (2017). In vitro and in vivo antifungal profile of a novel and long acting inhaled azole, PC945, on Aspergillus fumigatus infection. Antimicrobial Agents and Chemotherapy, AAC.02280-16
https://doi.org/10.1128/AAC.02280-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32189
Sewell, T., Hawkins, N., Stotz, H., Huang, Y., Kelly, S., Kelly, D., Fraaije, B., & Fitt, B. (2017). Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase. Scientific Reports, 7(1)
https://doi.org/10.1038/s41598-017-15545-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36850
Warrilow, A., Parker, J., Price, C., Nes, W., Garvey, E., Hoekstra, W., Schotzinger, R., Kelly, D., & Kelly, S. (2016). The Investigational Drug VT-1129 Is a Highly Potent Inhibitor of Cryptococcus Species CYP51 but Only Weakly Inhibits the Human Enzyme. Antimicrobial Agents and Chemotherapy, 60(8), 4530-4538.
https://doi.org/10.1128/AAC.00349-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29154
Warrilow, A., Price, C., Parker, J., Rolley, N., Smyrniotis, C., Hughes, D., Thoss, V., Nes, W., Kelly, D., Holman, T., & Kelly, S. (2016). Azole Antifungal Sensitivity of Sterol 14α-Demethylase (CYP51) and CYP5218 from Malassezia globosa. Scientific Reports, 6(1)
https://doi.org/10.1038/srep27690, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34335
Warrilow, A., Parker, J., Price, C., Nes, W., Kelly, S., & Kelly, D. (2015). In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus. Antimicrobial Agents and Chemotherapy, 59(12), 7771-7778.
https://doi.org/10.1128/aac.01806-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa23614
http://dx.doi.org/10.1128/aac.01806-15
Hull, C., Loveridge, J., Donnison, I., Kelly, D., & Kelly, S. (2014). Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept. AMB Express, 4(1), 64
https://doi.org/10.1186/s13568-014-0064-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18763
Hull, C., Loveridge, J., Rolley, N., Donnison, I., Kelly, S., & Kelly, D. (2014). Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock. Biotechnology for Biofuels, 7(1), 133
https://doi.org/10.1186/s13068-014-0133-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18617
Warrilow, A., Hull, C., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2014). Clotrimazole as a Potent Agent for Treating the Oomycete Fish Pathogen Saprolegnia parasitica through Inhibition of Sterol 14 -Demethylase (CYP51). Applied and Environmental Microbiology, 80(19), 6154-6166.
https://doi.org/10.1128/AEM.01195-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18616
Giaever, G., Chu, A., Ni, L., Connelly, C., Riles, L., Véronneau, S., Dow, S., Lucau-Danila, A., Anderson, K., André, B., Arkin, A., Astromoff, A., El Bakkoury, M., Bangham, R., Benito, R., Brachat, S., Campanaro, S., Curtiss, M., Davis, K...., & Johnston, M. (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418(6896), 387-391.
https://doi.org/10.1038/nature00935, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1332
Jackson, C. & Kelly, D. (2002). A Novel Sterol 14alpha -Demethylase/Ferredoxin Fusion Protein (MCCYP51FX) from Methylococcus capsulatus Represents a New Class of the Cytochrome P450 Superfamily. Journal of Biological Chemistry, 277(49), 46959-46965.
https://doi.org/10.1074/jbc.M203523200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1333
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200
Lamb, D., Jackson, C., Warrilow, A., Manning, N., Kelly, D., Kelly, S., & Kelly, D. (2007). Lanosterol Biosynthesis in the Prokaryote Methylococcus Capsulatus: Insight into the Evolution of Sterol Biosynthesis. Molecular Biology and Evolution, 24(8), 1714-1721.
https://doi.org/10.1093/molbev/msm090, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1335
Martel, C., Parker, J., Warrilow, A., & Kelly, D. (2010). Complementation of Saccharomyces cerevisiae ERG11/CYP51 regulated mutant and screening the azole sensitivity of Aspergillus fumigatus CYP51A and B.. Antimicrob Agents Chemother., 4920-4923.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9896
Kelly, D., Kraševec, N., Mullins, J., & Nelson, D. (2009). The CYPome (Cytochrome P450 complement) of Aspergillus nidulans. Fungal Genetics and Biology, 46(1), S53-S61.
https://doi.org/10.1016/j.fgb.2008.08.010, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10172
Price, C., Parker, J., Warrilow, A., Kelly, D., & Kelly, S. (2015). Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens. Pest Management Science, n/a-n/a.
https://doi.org/10.1002/ps.4029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20922
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Price, C., Warrilow, A., Parker, J., Mullins, J., Nes, W., Kelly, D., Kelly, S., & Schottel, J. (2015). Novel Substrate Specificity and Temperature-Sensitive Activity of Mycosphaerella graminicola CYP51 Supported by the Native NADPH Cytochrome P450 Reductase. Applied and Environmental Microbiology, 81(10), 3379-3386.
https://doi.org/10.1128/AEM.03965-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20704
Warrilow, A., Hull, C., Parker, J., Garvey, E., Hoekstra, W., Moore, W., Schotzinger, R., Kelly, D., Kelly, S., & Hull, C. (2014). The Clinical Candidate VT-1161 Is a Highly Potent Inhibitor of Candida albicans CYP51 but Fails To Bind the Human Enzyme. Antimicrobial Agents and Chemotherapy, 58(12), 7121-7127.
https://doi.org/10.1128/AAC.03707-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18693
Hawkins, N., Cools, H., Sierotzki, H., Shaw, M., Knogge, W., Kelly, S., Kelly, D., & Fraaije, B. (2014). Paralog Re-Emergence: A Novel, Historically Contingent Mechanism in the Evolution of Antimicrobial Resistance. Molecular Biology and Evolution, 31(7), 1793-1802.
https://doi.org/10.1093/molbev/msu134, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18161
http://dx.doi.org/10.1093/molbev/msu134
Nie, D., Hoobler, E., Rai, G., Warrilow, A., Perry, S., Smyrniotis, C., Jadhav, A., Simeonov, A., Parker, J., Kelly, D., Maloney, D., Kelly, S., & Holman, T. (2013). Discovery of a Novel Dual Fungal CYP51/Human 5-Lipoxygenase Inhibitor: Implications for Anti-Fungal Therapy. PLoS ONE, 8(6)
https://doi.org/10.1371/journal.pone.0065928, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15178
Belič, A., Pompon, D., Monostory, K., Kelly, D., Kelly, S., & Rozman, D. (2013). An algorithm for rapid computational construction of metabolic networks: A cholesterol biosynthesis example. Computers in Biology and Medicine, 43(5), 471-480.
https://doi.org/10.1016/j.compbiomed.2013.02.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15174
http://dx.doi.org/10.1016/j.compbiomed.2013.02.017
Kelly, S. & Kelly, D. (2013). Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1612), 20120476-20120476.
https://doi.org/10.1098/rstb.2012.0476, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14006
Kelly, S., Kelly, D., Parker, J., & Warrilow, A. (2013). Azole Affinity of Sterol 14alpha-Demethylase (CYP51) Enzymes from Candida albicans and Homo sapiens. Antimicrobial Agents and Chemotherapy, 57(3)-1360.
https://doi.org/10.1128/AAC.02067-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14415
Hull, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation inERG11and Exhibiting Cross-Resistance to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 56(8), 4223-4232.
https://doi.org/10.1128/aac.06253-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12338
http://dx.doi.org/10.1128/aac.06253-11
Mullins, J., Parker, J., Cools, H., Togawa, R., Lucas, J., Fraaije, B., Kelly, D., & Kelly, S. (2011). Molecular Modelling of the Emergence of Azole Resistance in Mycosphaerella graminicola. PLoS ONE, 6(6), e20973
https://doi.org/10.1371/journal.pone.0020973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6839
Cools, H., Mullins, J., Fraaije, B., Parker, J., Kelly, D., Lucas, J., & Kelly, S. (2011). Impact of Recently Emerged Sterol 14 alpha-Demethylase (CYP51) Variants of Mycosphaerella graminicola on Azole Fungicide Sensitivity. Applied and Environmental Microbiology, 77(11), 3830-3837.
https://doi.org/10.1128/AEM.00027-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6846
Martel, C., Parker, J., Jackson, C., Warrilow, A., Rolley, N., Greig, C., Morris, S., Donnison, I., Kelly, D., Kelly, S., & Hull, C. (2011). Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol. Bioresource Technology, 102(2), 1503-1508.
https://doi.org/10.1016/j.biortech.2010.07.099, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6360
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., Kelly, S., Parker, J., & Hull, C. (2010). A Clinical Isolate of Candida albicans with Mutations in ERG11 (Encoding Sterol 14alpha-Demethylase) and ERG5 (Encoding C22 Desaturase) Is Cross Resistant to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 54(9), 3578-3583.
https://doi.org/10.1128/AAC.00303-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6353
Warrilow, A., Melo, N., Martel, C., Parker, J., Nes, W., Kelly, S., & Kelly, D. (2010). Expression, Purification, and Characterization of Aspergillus fumigatus Sterol 14alpha-Demethylase (CYP51) Isoenzymes A and B. Antimicrobial Agents and Chemotherapy, 54(10), 4225-4234.
https://doi.org/10.1128/AAC.00316-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6354
Warrilow, A., Martel, C., Parker, J., Melo, N., Lamb, D., Nes, W., Kelly, D., Kelly, S., & Hull, C. (2010). Azole Binding Properties of Candida albicans Sterol 14alpha-Demethylase (CaCYP51). Antimicrobial Agents and Chemotherapy, 54(10), 4235-4245.
https://doi.org/10.1128/AAC.00587-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6355
Martel, C., Warrilow, A., Jackson, C., Mullins, J., Togawa, R., Parker, J., Morris, M., Donnison, I., Kelly, D., Kelly, S., Mullins, J., & Hull, C. (2010). Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans. Bioresource Technology, 101(12), 4395-4402.
https://doi.org/10.1016/j.biortech.2010.01.084, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6359
Parker, J., Warrilow, A., Cools, H., Martel, C., Nes, W., Fraaije, B., Lucas, J., Kelly, D., Kelly, S., & Hull, C. (2010). Mechanism of Binding of Prothioconazole to Mycosphaerella graminicola CYP51 Differs from That of Other Azole Antifungals. Applied and Environmental Microbiology, 77(4), 1460-1465.
https://doi.org/10.1128/AEM.01332-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6361
Cheng, Q., Lamb, D., Kelly, S., Lei, L., Guenguerich, F., & Kelly, D. (2010). Cyclization of a Cellular Dipentaenone by Streptomyces coelicolor Cytochrome P450 154A1 without Oxidation/Reduction. Journal of the American Chemical Society, 132(43), 15173-15175.
https://doi.org/10.1021/ja107801v, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9901
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Rolley, N., Kelly, D., Kelly, S., & Hull, C. (2010). Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans. Antimicrobial Agents and Chemotherapy, 54(11), 4527-4533.
https://doi.org/10.1128/AAC.00348-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6358
Cools, H., Parker, J., Kelly, D., Lucas, J., Fraaije, B., & Kelly, S. (2010). Heterologous Expression of Mutated Eburicol 14α-Demethylase (CYP51) Proteins of Mycosphaerella graminicola To Assess Effects on Azole Fungicide Sensitivity and Intrinsic Protein Function. Applied and Environmental Microbiology, 76(9), 2866-2872.
https://doi.org/10.1128/aem.02158-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6856
http://dx.doi.org/10.1128/aem.02158-09
Parker, J., Merkamm, M., Manning, N., Pompon, D., Kelly, S., & Kelly, D. (2008). Differential Azole Antifungal Efficacies Contrasted Using a Saccharomyces cerevisiae Strain Humanized for Sterol 14 alpha-Demethylase at the Homologous Locus. Antimicrobial Agents and Chemotherapy, 52(10), 3597-3603.
https://doi.org/10.1128/AAC.00517-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6835
Hull, C., Bader, O., Parker, J., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Two Clinical Isolates of Candida glabrata Exhibiting Reduced Sensitivity to Amphotericin B Both Harbor Mutations inERG2. Antimicrobial Agents and Chemotherapy, 56(12), 6417-6421.
https://doi.org/10.1128/aac.01145-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13028
http://dx.doi.org/10.1128/aac.01145-12
Binjubair, F., Parker, J., Warrilow, A., Puri, K., Braidley, P., Tatar, E., Kelly, S., Kelly, D., & Simons, C. (2020). Small‐Molecule Inhibitors Targeting Sterol 14α‐Demethylase (CYP51): Synthesis, Molecular Modelling and Evaluation Against Candida albicans. ChemMedChem, 15(14), 1294-1309.
https://doi.org/10.1002/cmdc.202000250, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54878
Price, C., Warrilow, A., Rolley, N., Parker, J., Thoss, V., Kelly, D., Corcionivoschi, N., & Kelly, S. (2022). Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. PLOS ONE, 17(3), e0265227
https://doi.org/10.1371/journal.pone.0265227, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59697
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64804
http://dx.doi.org/10.1186/s13068-017-0904-z

Holl Ymchwil

Erthyglau Cylchgronau

Price, C., Warrilow, A., Rolley, N., Parker, J., Thoss, V., Kelly, D., Corcionivoschi, N., & Kelly, S. (2022). Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. PLOS ONE, 17(3), e0265227
https://doi.org/10.1371/journal.pone.0265227, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59697
Binjubair, F., Parker, J., Warrilow, A., Puri, K., Braidley, P., Tatar, E., Kelly, S., Kelly, D., & Simons, C. (2020). Small‐Molecule Inhibitors Targeting Sterol 14α‐Demethylase (CYP51): Synthesis, Molecular Modelling and Evaluation Against Candida albicans. ChemMedChem, 15(14), 1294-1309.
https://doi.org/10.1002/cmdc.202000250, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54878
Warrilow, A., Parker, J., Price, C., Rolley, N., Nes, W., Kelly, D., & Kelly, S. (2019). Isavuconazole and voriconazole inhibition of sterol 14α-demethylases (CYP51) from Aspergillus fumigatus and Homo sapiens. International Journal of Antimicrobial Agents
https://doi.org/10.1016/j.ijantimicag.2019.07.011, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51096
Plácido, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology, 196, 106163
https://doi.org/10.1016/j.fuproc.2019.106163, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa51273
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment, 689, 10-20.
https://doi.org/10.1016/j.scitotenv.2019.06.390, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50905
Plácido, J., López, S., Meissner, K., Kelly, D., Kelly, S., & Placido Escobar, J. (2019). Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment, 688, 751-761.
https://doi.org/10.1016/j.scitotenv.2019.06.342, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50904
Placido Escobar, J., Bustamante-López, S., Meissner, K., Kelly, D., & Kelly, S. (2019). Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment, 656, 531-539.
https://doi.org/10.1016/j.scitotenv.2018.11.393, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46109
Griffiths, W., Crick, P., Meljon, A., Theofilopoulos, S., Abdel-Khalik, J., Yutuc, E., Parker, J., Kelly, D., Kelly, S., Arenas, E., & Wang, Y. (2019). Additional pathways of sterol metabolism: Evidence from analysis of Cyp27a1−/− mouse brain and plasma. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(2), 191-211.
https://doi.org/10.1016/j.bbalip.2018.11.006, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa46057
http://dx.doi.org/10.1016/j.bbalip.2018.11.006
Warrilow, A., Nishimoto, A., Parker, J., Price, C., Flowers, S., Kelly, D., Rogers, P., & Kelly, S. (2019). The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions. Antimicrobial Agents and Chemotherapy, 63(5)
https://doi.org/10.1128/aac.02586-18, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48946
Zhou, W., Warrilow, A., Thomas, C., Ramos, E., Parker, J., Price, C., Vanderloop, B., Fisher, P., Loftis, M., Kelly, D., Kelly, S., & Nes, W. (2018). Functional importance for developmental regulation of sterol biosynthesis in Acanthamoeba castellanii. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1863(10), 1164-1178.
https://doi.org/10.1016/j.bbalip.2018.07.004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa41148
Colley, T., Sehra, G., Chowdhary, A., Alanio, A., Kelly, S., Kizawa, Y., Armstrong-James, D., Fisher, M., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Nishimoto, Y., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J...., & Rapeport, G. (2018). In Vitro and In Vivo Efficacy of a Novel and Long-Acting Fungicidal Azole, PC1244, on Aspergillus fumigatus Infection. Antimicrobial Agents and Chemotherapy, 62(5), AAC.01941-17
https://doi.org/10.1128/aac.01941-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa38876
http://dx.doi.org/10.1128/aac.01941-17
Sewell, T., Hawkins, N., Stotz, H., Huang, Y., Kelly, S., Kelly, D., Fraaije, B., & Fitt, B. (2017). Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase. Scientific Reports, 7(1)
https://doi.org/10.1038/s41598-017-15545-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa36850
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa64804
http://dx.doi.org/10.1186/s13068-017-0904-z
Hull, C., Warrilow, A., Rolley, N., Price, C., Donnison, I., Kelly, D., & Kelly, S. (2017). Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. Biotechnology for Biofuels, 10(1)
https://doi.org/10.1186/s13068-017-0904-z, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37567
Warrilow, A., Parker, J., Price, C., Garvey, E., Hoekstra, W., Schotzinger, R., Wiederhold, N., Nes, W., Kelly, D., & Kelly, S. (2017). The Tetrazole VT-1161 Is a Potent Inhibitor of Trichophyton rubrum through Its Inhibition of T. rubrum CYP51. Antimicrobial Agents and Chemotherapy, 61(7), e00333-17
https://doi.org/10.1128/AAC.00333-17, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34176
Colley, T., Alanio, A., Kelly, S., Sehra, G., Kizawa, Y., Warrilow, A., Parker, J., Kelly, D., Kimura, G., Anderson-Dring, L., Nakaoki, T., Sunose, M., Onions, S., Crepin, D., Lagasse, F., Crittall, M., Shannon, J., Cooke, M., Bretagne, S...., & Rapeport, G. (2017). In vitro and in vivo antifungal profile of a novel and long acting inhaled azole, PC945, on Aspergillus fumigatus infection. Antimicrobial Agents and Chemotherapy, AAC.02280-16
https://doi.org/10.1128/AAC.02280-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa32189
Warrilow, A., Price, C., Parker, J., Rolley, N., Smyrniotis, C., Hughes, D., Thoss, V., Nes, W., Kelly, D., Holman, T., & Kelly, S. (2016). Azole Antifungal Sensitivity of Sterol 14α-Demethylase (CYP51) and CYP5218 from Malassezia globosa. Scientific Reports, 6(1)
https://doi.org/10.1038/srep27690, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34335
Warrilow, A., Parker, J., Price, C., Nes, W., Garvey, E., Hoekstra, W., Schotzinger, R., Kelly, D., & Kelly, S. (2016). The Investigational Drug VT-1129 Is a Highly Potent Inhibitor of Cryptococcus Species CYP51 but Only Weakly Inhibits the Human Enzyme. Antimicrobial Agents and Chemotherapy, 60(8), 4530-4538.
https://doi.org/10.1128/AAC.00349-16, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29154
Price, C., Parker, J., Warrilow, A., Kelly, D., & Kelly, S. (2015). Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens. Pest Management Science, n/a-n/a.
https://doi.org/10.1002/ps.4029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20922
Warrilow, A., Parker, J., Price, C., Nes, W., Kelly, S., & Kelly, D. (2015). In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus. Antimicrobial Agents and Chemotherapy, 59(12), 7771-7778.
https://doi.org/10.1128/aac.01806-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa23614
http://dx.doi.org/10.1128/aac.01806-15
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Price, C., Warrilow, A., Parker, J., Mullins, J., Nes, W., Kelly, D., Kelly, S., & Schottel, J. (2015). Novel Substrate Specificity and Temperature-Sensitive Activity of Mycosphaerella graminicola CYP51 Supported by the Native NADPH Cytochrome P450 Reductase. Applied and Environmental Microbiology, 81(10), 3379-3386.
https://doi.org/10.1128/AEM.03965-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa20704
Salvage, R., Hull, C., Kelly, D., & Kelly, S. (2014). Use of 70% alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms. Future Microbiology, 9(10), 1123-1130.
https://doi.org/10.2217/fmb.14.73, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa19624
Parker, J., Warrilow, A., Price, C., Mullins, J., Kelly, D., & Kelly, S. (2014). Resistance to antifungals that target CYP51. Journal of Chemical Biology, 7(4), 143-161.
https://doi.org/10.1007/s12154-014-0121-1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18694
Warrilow, A., Hull, C., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2014). Clotrimazole as a Potent Agent for Treating the Oomycete Fish Pathogen Saprolegnia parasitica through Inhibition of Sterol 14 -Demethylase (CYP51). Applied and Environmental Microbiology, 80(19), 6154-6166.
https://doi.org/10.1128/AEM.01195-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18616
Warrilow, A., Hull, C., Parker, J., Garvey, E., Hoekstra, W., Moore, W., Schotzinger, R., Kelly, D., Kelly, S., & Hull, C. (2014). The Clinical Candidate VT-1161 Is a Highly Potent Inhibitor of Candida albicans CYP51 but Fails To Bind the Human Enzyme. Antimicrobial Agents and Chemotherapy, 58(12), 7121-7127.
https://doi.org/10.1128/AAC.03707-14, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18693
Hull, C., Loveridge, J., Rolley, N., Donnison, I., Kelly, S., & Kelly, D. (2014). Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock. Biotechnology for Biofuels, 7(1), 133
https://doi.org/10.1186/s13068-014-0133-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18617
Hull, C., Loveridge, J., Donnison, I., Kelly, D., & Kelly, S. (2014). Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept. AMB Express, 4(1), 64
https://doi.org/10.1186/s13568-014-0064-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18763
Hawkins, N., Cools, H., Sierotzki, H., Shaw, M., Knogge, W., Kelly, S., Kelly, D., & Fraaije, B. (2014). Paralog Re-Emergence: A Novel, Historically Contingent Mechanism in the Evolution of Antimicrobial Resistance. Molecular Biology and Evolution, 31(7), 1793-1802.
https://doi.org/10.1093/molbev/msu134, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18161
http://dx.doi.org/10.1093/molbev/msu134
Nie, D., Hoobler, E., Rai, G., Warrilow, A., Perry, S., Smyrniotis, C., Jadhav, A., Simeonov, A., Parker, J., Kelly, D., Maloney, D., Kelly, S., & Holman, T. (2013). Discovery of a Novel Dual Fungal CYP51/Human 5-Lipoxygenase Inhibitor: Implications for Anti-Fungal Therapy. PLoS ONE, 8(6)
https://doi.org/10.1371/journal.pone.0065928, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15178
Kelly, S. & Kelly, D. (2013). Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1612), 20120476-20120476.
https://doi.org/10.1098/rstb.2012.0476, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14006
Belič, A., Pompon, D., Monostory, K., Kelly, D., Kelly, S., & Rozman, D. (2013). An algorithm for rapid computational construction of metabolic networks: A cholesterol biosynthesis example. Computers in Biology and Medicine, 43(5), 471-480.
https://doi.org/10.1016/j.compbiomed.2013.02.017, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa15174
http://dx.doi.org/10.1016/j.compbiomed.2013.02.017
Kelly, S., Kelly, D., Parker, J., & Warrilow, A. (2013). Azole Affinity of Sterol 14alpha-Demethylase (CYP51) Enzymes from Candida albicans and Homo sapiens. Antimicrobial Agents and Chemotherapy, 57(3)-1360.
https://doi.org/10.1128/AAC.02067-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14415
Warrilow, A., Mullins, J., Hull, C., Parker, J., Lamb, D., Kelly, D., Kelly, S., & Mullins, J. (2012). S279 Point Mutations in Candida albicans Sterol 14- Demethylase (CYP51) Reduce In Vitro Inhibition by Fluconazole. Antimicrobial Agents and Chemotherapy, 56(4), 2099-2107.
https://doi.org/10.1128/AAC.05389-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa14242
Hull, C., Bader, O., Parker, J., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Two Clinical Isolates of Candida glabrata Exhibiting Reduced Sensitivity to Amphotericin B Both Harbor Mutations inERG2. Antimicrobial Agents and Chemotherapy, 56(12), 6417-6421.
https://doi.org/10.1128/aac.01145-12, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa13028
http://dx.doi.org/10.1128/aac.01145-12
Hull, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., & Kelly, S. (2012). Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation inERG11and Exhibiting Cross-Resistance to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 56(8), 4223-4232.
https://doi.org/10.1128/aac.06253-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa12338
http://dx.doi.org/10.1128/aac.06253-11
Mullins, J., Parker, J., Cools, H., Togawa, R., Lucas, J., Fraaije, B., Kelly, D., & Kelly, S. (2011). Molecular Modelling of the Emergence of Azole Resistance in Mycosphaerella graminicola. PLoS ONE, 6(6), e20973
https://doi.org/10.1371/journal.pone.0020973, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6839
Cools, H., Mullins, J., Fraaije, B., Parker, J., Kelly, D., Lucas, J., & Kelly, S. (2011). Impact of Recently Emerged Sterol 14 alpha-Demethylase (CYP51) Variants of Mycosphaerella graminicola on Azole Fungicide Sensitivity. Applied and Environmental Microbiology, 77(11), 3830-3837.
https://doi.org/10.1128/AEM.00027-11, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6846
Martel, C., Parker, J., Jackson, C., Warrilow, A., Rolley, N., Greig, C., Morris, S., Donnison, I., Kelly, D., Kelly, S., & Hull, C. (2011). Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol. Bioresource Technology, 102(2), 1503-1508.
https://doi.org/10.1016/j.biortech.2010.07.099, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6360
Martel, C., Parker, J., Warrilow, A., Rolley, N., Kelly, S., Kelly, D., Parker, J., Hull, C., & Warrilow, A. (2010). Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14alpha-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B. Antimicrobial Agents and Chemotherapy, 54(11), 4920-4923.
https://doi.org/10.1128/AAC.00349-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6356
Martel, C., Parker, J., Warrilow, A., & Kelly, D. (2010). Complementation of Saccharomyces cerevisiae ERG11/CYP51 regulated mutant and screening the azole sensitivity of Aspergillus fumigatus CYP51A and B.. Antimicrob Agents Chemother., 4920-4923.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9896
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Kelly, D., Kelly, S., Parker, J., & Hull, C. (2010). A Clinical Isolate of Candida albicans with Mutations in ERG11 (Encoding Sterol 14alpha-Demethylase) and ERG5 (Encoding C22 Desaturase) Is Cross Resistant to Azoles and Amphotericin B. Antimicrobial Agents and Chemotherapy, 54(9), 3578-3583.
https://doi.org/10.1128/AAC.00303-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6353
Warrilow, A., Melo, N., Martel, C., Parker, J., Nes, W., Kelly, S., & Kelly, D. (2010). Expression, Purification, and Characterization of Aspergillus fumigatus Sterol 14alpha-Demethylase (CYP51) Isoenzymes A and B. Antimicrobial Agents and Chemotherapy, 54(10), 4225-4234.
https://doi.org/10.1128/AAC.00316-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6354
Warrilow, A., Martel, C., Parker, J., Melo, N., Lamb, D., Nes, W., Kelly, D., Kelly, S., & Hull, C. (2010). Azole Binding Properties of Candida albicans Sterol 14alpha-Demethylase (CaCYP51). Antimicrobial Agents and Chemotherapy, 54(10), 4235-4245.
https://doi.org/10.1128/AAC.00587-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6355
Martel, C., Warrilow, A., Jackson, C., Mullins, J., Togawa, R., Parker, J., Morris, M., Donnison, I., Kelly, D., Kelly, S., Mullins, J., & Hull, C. (2010). Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans. Bioresource Technology, 101(12), 4395-4402.
https://doi.org/10.1016/j.biortech.2010.01.084, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6359
Parker, J., Warrilow, A., Cools, H., Martel, C., Nes, W., Fraaije, B., Lucas, J., Kelly, D., Kelly, S., & Hull, C. (2010). Mechanism of Binding of Prothioconazole to Mycosphaerella graminicola CYP51 Differs from That of Other Azole Antifungals. Applied and Environmental Microbiology, 77(4), 1460-1465.
https://doi.org/10.1128/AEM.01332-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6361
Cheng, Q., Lamb, D., Kelly, S., Lei, L., Guenguerich, F., & Kelly, D. (2010). Cyclization of a Cellular Dipentaenone by Streptomyces coelicolor Cytochrome P450 154A1 without Oxidation/Reduction. Journal of the American Chemical Society, 132(43), 15173-15175.
https://doi.org/10.1021/ja107801v, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa9901
Martel, C., Parker, J., Bader, O., Weig, M., Gross, U., Warrilow, A., Rolley, N., Kelly, D., Kelly, S., & Hull, C. (2010). Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans. Antimicrobial Agents and Chemotherapy, 54(11), 4527-4533.
https://doi.org/10.1128/AAC.00348-10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6358
Cools, H., Parker, J., Kelly, D., Lucas, J., Fraaije, B., & Kelly, S. (2010). Heterologous Expression of Mutated Eburicol 14α-Demethylase (CYP51) Proteins of Mycosphaerella graminicola To Assess Effects on Azole Fungicide Sensitivity and Intrinsic Protein Function. Applied and Environmental Microbiology, 76(9), 2866-2872.
https://doi.org/10.1128/aem.02158-09, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6856
http://dx.doi.org/10.1128/aem.02158-09
Bader, O., Kuhns, M., Hull, C., Parker, J., Bader, M., Tintelnot, K., Seiboldt, M., Kelly, D., Kelly, S., Gross, U., & Weig, M. (2009). Frequency of azole resistance phenotypes in the two most prevalent human pathogenic yeast, Candida albicans and Candida glabrata. MYCOSES, 52(5)-421.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa16023
Kelly, D., Kraševec, N., Mullins, J., & Nelson, D. (2009). The CYPome (Cytochrome P450 complement) of Aspergillus nidulans. Fungal Genetics and Biology, 46(1), S53-S61.
https://doi.org/10.1016/j.fgb.2008.08.010, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10172
Warrilow, A., Ugochukwu, C., Lamb, D., Kelly, D., & Kelly, S. (2008). Expression and Characterization of CYP51, the Ancient Sterol 14alpha-demethylase Activity for Cytochromes P450 (CYP), in the White-Rot Fungus Phanerochaete chrysosporium. Lipids, 43(12), 1143-1153.
https://doi.org/10.1007/s11745-008-3239-5, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6350
Warrilow, A., Jackson, C., Parker, J., Marczylo, T., Kelly, D., Lamb, D., & Kelly, S. (2008). Identification, Characterization, and Azole-Binding Properties of Mycobacterium smegmatis CYP164A2, a Homolog of ML2088, the Sole Cytochrome P450 Gene of Mycobacterium leprae. Antimicrobial Agents and Chemotherapy, 53(3), 1157-1164.
https://doi.org/10.1128/AAC.01237-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6351
Melo, N., Moran, G., Warrilow, A., Dudley, E., Smith, S., Sullivan, D., Lamb, D., Kelly, D., Coleman, D., & Kelly, S. (2008). CYP56 (Dit2p) in Candida albicans: Characterization and Investigation of Its Role in Growth and Antifungal Drug Susceptibility. Antimicrobial Agents and Chemotherapy, 52(10), 3718-3724.
https://doi.org/10.1128/AAC.00446-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6349
Parker, J., Merkamm, M., Manning, N., Pompon, D., Kelly, S., & Kelly, D. (2008). Differential Azole Antifungal Efficacies Contrasted Using a Saccharomyces cerevisiae Strain Humanized for Sterol 14 alpha-Demethylase at the Homologous Locus. Antimicrobial Agents and Chemotherapy, 52(10), 3597-3603.
https://doi.org/10.1128/AAC.00517-08, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6835
Lamb, D., Jackson, C., Warrilow, A., Manning, N., Kelly, D., Kelly, S., & Kelly, D. (2007). Lanosterol Biosynthesis in the Prokaryote Methylococcus Capsulatus: Insight into the Evolution of Sterol Biosynthesis. Molecular Biology and Evolution, 24(8), 1714-1721.
https://doi.org/10.1093/molbev/msm090, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1335
Barber, R., Hickenbotham, P., Hatch, T., Kelly, D., Topchiy, N., Almeida, G., Jones, G., Johnson, G., Parry, J., Rothkamm, K., Dubrova, Y., & Johnson, G. (2006). Radiation-induced transgenerational alterations in genome stability and DNA damage. Oncogene, 25(56), 7336-7342.
https://doi.org/10.1038/sj.onc.1209723, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa17857
Jackson, C., Lamb, D., Marczylo, T., Parker, J., Manning, N., Kelly, D., & Kelly, S. (2003). Conservation and cloning of CYP51: a sterol 14α-demethylase from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications, 301(2), 558
https://doi.org/10.1016/S0006-291X(02)03078-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa6859
Giaever, G., Chu, A., Ni, L., Connelly, C., Riles, L., Véronneau, S., Dow, S., Lucau-Danila, A., Anderson, K., André, B., Arkin, A., Astromoff, A., El Bakkoury, M., Bangham, R., Benito, R., Brachat, S., Campanaro, S., Curtiss, M., Davis, K...., & Johnston, M. (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418(6896), 387-391.
https://doi.org/10.1038/nature00935, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1332
Jackson, C. & Kelly, D. (2002). A Novel Sterol 14alpha -Demethylase/Ferredoxin Fusion Protein (MCCYP51FX) from Methylococcus capsulatus Represents a New Class of the Cytochrome P450 Superfamily. Journal of Biological Chemistry, 277(49), 46959-46965.
https://doi.org/10.1074/jbc.M203523200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1333
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200
Lamb, D., Kaderbhai, N., Venkateswarlu, K., Kelly, D., Kelly, S., Kaderbhai, M., & Kanamarlapudi, V. (2001). Human Sterol 14α-Demethylase Activity Is Enhanced by the Membrane-Bound State of Cytochrome b5. Archives of Biochemistry and Biophysics, 395(1), 78-84.
https://doi.org/10.1006/abbi.2001.2566, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25098
Lamb, D., Warrilow, A., Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (2001). Activities and Kinetic Mechanisms of Native and Soluble NADPH–Cytochrome P450 Reductase. Biochemical and Biophysical Research Communications, 286(1), 48-54.
https://doi.org/10.1006/bbrc.2001.5338, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25100
Lamb, D., Kelly, D., Venkateswarlu, K., Manning, N., Bligh, H., Schunck, W., Kelly, S., & Kanamarlapudi, V. (1999). Generation of a Complete, Soluble, and Catalytically Active Sterol 14α-Demethylase−Reductase Complex†. Biochemistry, 38(27), 8733-8738.
https://doi.org/10.1021/bi9825089, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25106
Venkateswarlu, K., Kelly, D., Manning, N., Kelly, S., & Kanamarlapudi, V. (1998). NADPH cytochrome P-450 oxidoreductase and susceptibility to ketoconazole. Antimicrobial Agents and Chemotherapy, 42
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25111
Venkateswarlu, K., Lamb, D., Kelly, D., Manning, N., Kelly, S., & Kanamarlapudi, V. (1998). The N-Terminal Membrane Domain of Yeast NADPH-Cytochrome P450 (CYP) Oxidoreductase Is Not Required for Catalytic Activity in Sterol Biosynthesis or in Reconstitution of CYP Activity. Journal of Biological Chemistry, 273(8), 4492-4496.
https://doi.org/10.1074/jbc.273.8.4492, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25109
Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (1997). Characterization of Saccharomyces cerevisiae CYP51 and a CYP51 fusion protein with NADPH cytochrome P-450 oxidoreductase expressed in Escherichia coli. Antimicrobial Agents and Chemotherapy, 41
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25119
Fang, G., Venkateswarlu, K., Kelly, D., Kelly, S., & Kanamarlapudi, V. (1994). STUDIES ON YEAST HOST STRAINS FOR HETEROLOGOUS P450 EXPRESSION. Biotechnology Letters, 16
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa25127

Grantiau a Phrosiectau Allweddol

Bio-refining Centre of Excellence: BEACON 2010 - 2023

, with Prifysgol Aberystwyth; Prifysgol Bangor; Prifysgol De Cymru, £18.3 miliwn
BBSRC-FAPESP Newton Fund Award: Application of novel real-time disease surveillance and fungicide resistance monitoring tools to foster a smart and sustainable crop protection platform in Brazil 2019 - 2021

, with Rothamsted Research, Universidade Estadual Paulista “Julio de Mesquita”, San Paulo Brazil, £365,101