Institute of Life Science 1 internal Atrium view up

Dr Stephen Evans

Swyddog Ymchwil

Biomedical Sciences

+44 (0) 1792 205678 ext 1501

s.j.evans@abertawe.ac.uk

038
Llawr Gwaelod
Y Sefydliad Gwyddor Bywyd 1
Campws Singleton

Ar gael ar gyfer Goruchwyliaeth Ôl-raddedig

Dolenni Ymchwil

https://orcid.org/0000-0002-5352-9800

Trosolwg

Enillodd Stephen ei PhD mewn gwenwyneg enetig o Brifysgol Abertawe yn 2016; canolbwyntiodd ei draethawd ymchwil ar ddatblygu modelau cyd-ddiwylliant vitroairway ar gyfer asesu genotocsigrwydd nanoronynnau.

Mae Stephen bellach yn Swyddog Ymchwil ac Arloesi o fewn Grwp VitroToxicology (IVTG) yn Abertawe fel rhan o Rwydwaith Arloesi Gwyddorau Bywyd Celtaidd (CALIN) a ariennir gan Lywodraeth Cymru a'r UE. Mae ei rôl yn canolbwyntio ar gynorthwyo cwmnïau bach i ddatblygu cynnyrch a phrosesau. Mae prif ddiddordebau ymchwil Stephen mewn nanotoxicology, gwenwyneg genetig, modelau vitrocell datblygedig, nodweddu nanoronynnau a microsgopeg electronau.

Meysydd Arbenigedd

Nanotocsicoleg
Tocsicoleg Genetig
Rhyngweithiadau Nanoparticle-Cell
Systemau Cellog Uwch In Vitro
Microsgopeg Electron
Nodweddu Nanoparticle

Cyhoeddiadau

Uchafbwyntiau Ymchwil

Burgum, M., Ulrich, C., Partosa, N., Evans, S., Gomes, C., Seiffert, S., Landsiedel, R., Honarvar, N., & Doak, S. (2024). Adapting the in vitro micronucleus assay (OECD Test Guideline No. 487) for testing of manufactured nanomaterials: recommendations for best practices. Mutagenesis, 39(3), 205-217.
https://doi.org/10.1093/mutage/geae010, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65963
http://dx.doi.org/10.1093/mutage/geae010
Bannuscher, A., Schmid, O., Drasler, B., Rohrbasser, A., Braakhuis, H., Meldrum, K., Zwart, E., Gremmer, E., Birk, B., Rissel, M., Landsiedel, R., Moschini, E., Evans, S., Kumar, P., Orak, S., Doryab, A., Erdem, J., Serchi, T., Vandebriel, R...., & Rothen-Rutishauser, B. (2022). An inter-laboratory effort to harmonize the cell-delivered in vitro dose of aerosolized materials. NanoImpact, 28, 100439
https://doi.org/10.1016/j.impact.2022.100439, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62077
Brown, S., Evans, S., Burgum, M., Meldrum, K., Herridge, J., Akinbola, B., Harris, L., Jenkins, R., Doak, S., Clift, M., & Wilkinson, T. (2024). An In Vitro Model to Assess Early Immune Markers Following Co-Exposure of Epithelial Cells to Carbon Black (Nano)Particles in the Presence of S. aureus: A Role for Stressed Cells in Toxicological Testing. Biomedicines, 12(1), 128
https://doi.org/10.3390/biomedicines12010128, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65427
Evans, S., Lawrence, R., Ilett, M., Burgum, M., Meldrum, K., Hondow, N., Jenkins, G., Clift, M., & Doak, S. (2022). Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction. Toxicology in Vitro, 83, 105415
https://doi.org/10.1016/j.tiv.2022.105415, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60312
Llewellyn, S., Parak, W., Hühn, J., Burgum, M., Evans, S., Chapman, K., Jenkins, G., Doak, S., & Clift, M. (2022). Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines in vitro. Nanotoxicology, 16(1), 52-72.
https://doi.org/10.1080/17435390.2022.2030823, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59210
Meldrum, K., Evans, S., Vogel, U., Tran, L., Doak, S., & Clift, M. (2022). The influence of exposure approaches to in vitro lung epithelial barrier models to assess engineered nanomaterial hazard. Nanotoxicology, 16(1), 114-134.
https://doi.org/10.1080/17435390.2022.2051627, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59667
Evans, S., Jenkins, G., Doak, S., & Clift, M. (2019). Cellular Defense Mechanisms Following Nanomaterial Exposure: A Focus on Oxidative Stress and Cytotoxicity. Biological Responses to Nanoscale Particles (pp. 243-254). Springer International Publishing
https://doi.org/10.1007/978-3-030-12461-8_10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50127
Evans, S., Vecchiarelli, P., Clift, M., Doak, S., & Lead, J. (2021). Overview of Nanotoxicology in Humans and the Environment; Developments, Challenges and Impacts. Nanotoxicology in Humans and the Environment (pp. 1-40). Springer International Publishing
https://doi.org/10.1007/978-3-030-79808-6_1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59028
Groff, K., Evans, S., Doak, S., Pfuhler, S., Corvi, R., Saunders, S., & Stoddart, G. (2021). In vitro and integrated in vivo strategies to reduce animal use in genotoxicity testing. Mutagenesis, 36(6), 389-400.
https://doi.org/10.1093/mutage/geab035, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58118
Evans, S., Roberts, A., Morris, A., Simpson, A., Harris, L., Mack, D., Jenkins, R., & Wilkinson, T. (2020). Contrasting effects of linezolid on healthy and dysfunctional human neutrophils: reducing C5a-induced injury. Scientific Reports, 10(1)
https://doi.org/10.1038/s41598-020-72454-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55318
Evans, S., Gollapudi, B., Moore, M., & Doak, S. (2019). Horizon scanning for novel and emerging in vitro mammalian cell mutagenicity test systems. Mutation Research/Genetic Toxicology and Environmental Mutagenesis
https://doi.org/10.1016/j.mrgentox.2019.02.005, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49201
Llewellyn, S., Conway, G., Shah, U., Evans, S., Jenkins, G., Clift, M., & Doak, S. (2020). Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. Journal of Visualized Experiments(160)
https://doi.org/10.3791/61141, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54416
Burgum, M., Evans, S., Jenkins, G., Doak, S., & Clift, M. (2018). Considerations for the Human Health Implications of Nanotheranostics. Handbook of Nanomaterials for Cancer Theranostics (pp. 279-303). Elsevier
https://doi.org/10.1016/b978-0-12-813339-2.00010-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54455
http://dx.doi.org/10.1016/b978-0-12-813339-2.00010-4
Evans, S., Clift, M., Singh, N., Mallia, J., Burgum, M., Wills, J., Wilkinson, T., Jenkins, G., & Doak, S. (2017). Critical review of the current and future challenges associated with advancedin vitrosystems towards the study of nanoparticle (secondary) genotoxicity. Mutagenesis, 32(1), 233-241.
https://doi.org/10.1093/mutage/gew054, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54456
http://dx.doi.org/10.1093/mutage/gew054
Ruiz-de-Angulo, A., Bilbao Asensio, M., Cronin, J., Evans, S., Clift, M., Llop, J., Feiner, I., Beadman, R., Bascarán, K., & Mareque-Rivas, J. (2020). Chemically Programmed Vaccines: Iron Catalysis in Nanoparticles Enhances Combination Immunotherapy and Immunotherapy-Promoted Tumor Ferroptosis. iScience, 23(9), 101499
https://doi.org/10.1016/j.isci.2020.101499, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55075
Burgum, M., Clift, M., Evans, S., Hondow, N., Miller, M., Bustamante-Lopez, S., Williams, A., Tarat, A., Jenkins, G., & Doak, S. (2021). In Vitro Primary‐Indirect Genotoxicity in Bronchial Epithelial Cells Promoted by Industrially Relevant Few‐Layer Graphene. Small, 17(15), 2002551
https://doi.org/10.1002/smll.202002551, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54412
Conway, G., Shah, U., Llewellyn, S., Cervena, T., Evans, S., Al-Ali, A., Jenkins, G., Clift, M., & Doak, S. (2020). Adaptation of the in vitro micronucleus assay for genotoxicity testing using 3D liver models supporting longer-term exposure durations. Mutagenesis, 35(4), 319-329.
https://doi.org/10.1093/mutage/geaa018, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54991
Burgum, M., Clift, M., Evans, S., Hondow, N., Tarat, A., Jenkins, G., & Doak, S. (2021). Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro. Journal of Nanobiotechnology, 19(1)
https://doi.org/10.1186/s12951-021-00769-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56078
Evans, S., Clift, M., Singh, N., Wills, J., Hondow, N., Wilkinson, T., Burgum, M., Brown, A., Jenkins, G., & Doak, S. (2019). In vitro detection of in vitro secondary mechanisms of genotoxicity induced by engineered nanomaterials. Particle and Fibre Toxicology, 16(1)
https://doi.org/10.1186/s12989-019-0291-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48861
Evans, S., Clift, M., Singh, N., Wills, J., Hondow, N., Brown, A., Jenkins, G., Doak, S., & Clift, M. (2017). In vitro assessment of secondary genotoxic mechanisms induced by superparamagnetic iron oxide nanoparticles using airway models. In Mutagenesis (pp. 607-628).
https://doi.org/10.1093/mutage/gex033, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa39525
Thomson, E., Jessop, Z., Naderi, N., Griffin, M., Dobbs, T., Ibrahim, A., Evans, S., Burnell, S., Doak, S., & Whitaker, I. (2016). Adipose regeneration and implications for breast reconstruction: update and the future. Gland Surgery, 5(2), 227-241.
https://doi.org/10.3978/j.issn.2227-684X.2016.01.01, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56657
https://gs.amegroups.com/article/view/9095
Meldrum, K., Evans, S., Burgum, M., Doak, S., & Clift, M. (2024). Determining the toxicological effects of indoor air pollution on both a healthy and an inflammatory-comprised model of the alveolar epithelial barrier in vitro. Particle and Fibre Toxicology, 21(1)
https://doi.org/10.1186/s12989-024-00584-8, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66993

Holl Ymchwil

Erthyglau Cylchgronau

Meldrum, K., Evans, S., Burgum, M., Doak, S., & Clift, M. (2024). Determining the toxicological effects of indoor air pollution on both a healthy and an inflammatory-comprised model of the alveolar epithelial barrier in vitro. Particle and Fibre Toxicology, 21(1)
https://doi.org/10.1186/s12989-024-00584-8, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66993
Burgum, M., Ulrich, C., Partosa, N., Evans, S., Gomes, C., Seiffert, S., Landsiedel, R., Honarvar, N., & Doak, S. (2024). Adapting the in vitro micronucleus assay (OECD Test Guideline No. 487) for testing of manufactured nanomaterials: recommendations for best practices. Mutagenesis, 39(3), 205-217.
https://doi.org/10.1093/mutage/geae010, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65963
http://dx.doi.org/10.1093/mutage/geae010
Brown, S., Evans, S., Burgum, M., Meldrum, K., Herridge, J., Akinbola, B., Harris, L., Jenkins, R., Doak, S., Clift, M., & Wilkinson, T. (2024). An In Vitro Model to Assess Early Immune Markers Following Co-Exposure of Epithelial Cells to Carbon Black (Nano)Particles in the Presence of S. aureus: A Role for Stressed Cells in Toxicological Testing. Biomedicines, 12(1), 128
https://doi.org/10.3390/biomedicines12010128, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65427
Bannuscher, A., Schmid, O., Drasler, B., Rohrbasser, A., Braakhuis, H., Meldrum, K., Zwart, E., Gremmer, E., Birk, B., Rissel, M., Landsiedel, R., Moschini, E., Evans, S., Kumar, P., Orak, S., Doryab, A., Erdem, J., Serchi, T., Vandebriel, R...., & Rothen-Rutishauser, B. (2022). An inter-laboratory effort to harmonize the cell-delivered in vitro dose of aerosolized materials. NanoImpact, 28, 100439
https://doi.org/10.1016/j.impact.2022.100439, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62077
Evans, S., Lawrence, R., Ilett, M., Burgum, M., Meldrum, K., Hondow, N., Jenkins, G., Clift, M., & Doak, S. (2022). Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction. Toxicology in Vitro, 83, 105415
https://doi.org/10.1016/j.tiv.2022.105415, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60312
Meldrum, K., Evans, S., Vogel, U., Tran, L., Doak, S., & Clift, M. (2022). The influence of exposure approaches to in vitro lung epithelial barrier models to assess engineered nanomaterial hazard. Nanotoxicology, 16(1), 114-134.
https://doi.org/10.1080/17435390.2022.2051627, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59667
Llewellyn, S., Parak, W., Hühn, J., Burgum, M., Evans, S., Chapman, K., Jenkins, G., Doak, S., & Clift, M. (2022). Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines in vitro. Nanotoxicology, 16(1), 52-72.
https://doi.org/10.1080/17435390.2022.2030823, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59210
Groff, K., Evans, S., Doak, S., Pfuhler, S., Corvi, R., Saunders, S., & Stoddart, G. (2021). In vitro and integrated in vivo strategies to reduce animal use in genotoxicity testing. Mutagenesis, 36(6), 389-400.
https://doi.org/10.1093/mutage/geab035, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa58118
Burgum, M., Clift, M., Evans, S., Hondow, N., Miller, M., Bustamante-Lopez, S., Williams, A., Tarat, A., Jenkins, G., & Doak, S. (2021). In Vitro Primary‐Indirect Genotoxicity in Bronchial Epithelial Cells Promoted by Industrially Relevant Few‐Layer Graphene. Small, 17(15), 2002551
https://doi.org/10.1002/smll.202002551, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54412
Burgum, M., Clift, M., Evans, S., Hondow, N., Tarat, A., Jenkins, G., & Doak, S. (2021). Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro. Journal of Nanobiotechnology, 19(1)
https://doi.org/10.1186/s12951-021-00769-9, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56078
Evans, S., Roberts, A., Morris, A., Simpson, A., Harris, L., Mack, D., Jenkins, R., & Wilkinson, T. (2020). Contrasting effects of linezolid on healthy and dysfunctional human neutrophils: reducing C5a-induced injury. Scientific Reports, 10(1)
https://doi.org/10.1038/s41598-020-72454-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55318
Ruiz-de-Angulo, A., Bilbao Asensio, M., Cronin, J., Evans, S., Clift, M., Llop, J., Feiner, I., Beadman, R., Bascarán, K., & Mareque-Rivas, J. (2020). Chemically Programmed Vaccines: Iron Catalysis in Nanoparticles Enhances Combination Immunotherapy and Immunotherapy-Promoted Tumor Ferroptosis. iScience, 23(9), 101499
https://doi.org/10.1016/j.isci.2020.101499, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55075
Conway, G., Shah, U., Llewellyn, S., Cervena, T., Evans, S., Al-Ali, A., Jenkins, G., Clift, M., & Doak, S. (2020). Adaptation of the in vitro micronucleus assay for genotoxicity testing using 3D liver models supporting longer-term exposure durations. Mutagenesis, 35(4), 319-329.
https://doi.org/10.1093/mutage/geaa018, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54991
Llewellyn, S., Conway, G., Shah, U., Evans, S., Jenkins, G., Clift, M., & Doak, S. (2020). Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. Journal of Visualized Experiments(160)
https://doi.org/10.3791/61141, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54416
Evans, S., Gollapudi, B., Moore, M., & Doak, S. (2019). Horizon scanning for novel and emerging in vitro mammalian cell mutagenicity test systems. Mutation Research/Genetic Toxicology and Environmental Mutagenesis
https://doi.org/10.1016/j.mrgentox.2019.02.005, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa49201
Evans, S., Clift, M., Singh, N., Wills, J., Hondow, N., Wilkinson, T., Burgum, M., Brown, A., Jenkins, G., & Doak, S. (2019). In vitro detection of in vitro secondary mechanisms of genotoxicity induced by engineered nanomaterials. Particle and Fibre Toxicology, 16(1)
https://doi.org/10.1186/s12989-019-0291-7, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa48861
Evans, S., Clift, M., Singh, N., Mallia, J., Burgum, M., Wills, J., Wilkinson, T., Jenkins, G., & Doak, S. (2017). Critical review of the current and future challenges associated with advancedin vitrosystems towards the study of nanoparticle (secondary) genotoxicity. Mutagenesis, 32(1), 233-241.
https://doi.org/10.1093/mutage/gew054, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54456
http://dx.doi.org/10.1093/mutage/gew054
Thomson, E., Jessop, Z., Naderi, N., Griffin, M., Dobbs, T., Ibrahim, A., Evans, S., Burnell, S., Doak, S., & Whitaker, I. (2016). Adipose regeneration and implications for breast reconstruction: update and the future. Gland Surgery, 5(2), 227-241.
https://doi.org/10.3978/j.issn.2227-684X.2016.01.01, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa56657
https://gs.amegroups.com/article/view/9095

Penodau Llyfrau

Evans, S., Vecchiarelli, P., Clift, M., Doak, S., & Lead, J. (2021). Overview of Nanotoxicology in Humans and the Environment; Developments, Challenges and Impacts. Nanotoxicology in Humans and the Environment (pp. 1-40). Springer International Publishing
https://doi.org/10.1007/978-3-030-79808-6_1, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59028
Evans, S., Jenkins, G., Doak, S., & Clift, M. (2019). Cellular Defense Mechanisms Following Nanomaterial Exposure: A Focus on Oxidative Stress and Cytotoxicity. Biological Responses to Nanoscale Particles (pp. 243-254). Springer International Publishing
https://doi.org/10.1007/978-3-030-12461-8_10, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50127
Burgum, M., Evans, S., Jenkins, G., Doak, S., & Clift, M. (2018). Considerations for the Human Health Implications of Nanotheranostics. Handbook of Nanomaterials for Cancer Theranostics (pp. 279-303). Elsevier
https://doi.org/10.1016/b978-0-12-813339-2.00010-4, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54455
http://dx.doi.org/10.1016/b978-0-12-813339-2.00010-4

Cyfraniadau Cynadleddau

Evans, S., Clift, M., Singh, N., Wills, J., Hondow, N., Brown, A., Jenkins, G., Doak, S., & Clift, M. (2017). In vitro assessment of secondary genotoxic mechanisms induced by superparamagnetic iron oxide nanoparticles using airway models. In Mutagenesis (pp. 607-628).
https://doi.org/10.1093/mutage/gex033, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa39525

Modiwlau a Addysgir

PM-351 Nanotoxicology

Nanomaterials are comprised of very small particles that provide many of the products we use increased strength, chemical reactivity or conductivity. They can also be the by-product of e.g. industrial processes or they can occur naturally, as with soot from forest fires or volcanic ash. The particles range in size from approximately 1 to 100 nanometres; that is 1 / 1,000,000,000th of a metre. Due to their small size they can readily enter our bodies through a number of exposure routes, of which inhalation, ingestion or absorption through the skin, as well as injection into the circulation are dominant. These particles have unique properties compared to their larger counterparts and we are only beginning to understand what their health effects may be. The purpose of this module is to give an introduction into the field of nanoscience, nanotechnology and the potential risks posed by this new Industrial Age to humans.
PM-378 Biomedical Laboratory Techniques

The module will provide practical and in depth theory of applications and equipment available to MSci students in the biomedical research laboratories based at the Medical School. The module will provide guidelines and rationale for experimental design, and data and statistical analysis.
PM-378C Technegau Labordy Biofeddygol

Bydd y modiwl yn darparu damcaniaeth ymarferol a manwl o¿r cymwysiadau ac offer sydd ar gael i fyfyrwyr MSci yn y labordai ymchwil biofeddygol sydd wedi'u lleoli yn yr Ysgol Feddygaeth. Bydd y modiwl yn darparu canllawiau a rhesymeg ar gyfer cynlluniau arbrofol, a dadansoddiad data ac ystadegau.
PM-M28 Nano(geno)toxicology

The field of nanotechnology is ever increasing in size, and the number of nano-sized (1-100nm) particles being produced on a daily basis is at the tonnage level. Although nanotechnology-based applications are considered to be advantageous for a plethora of human activities, the potential risks posed by human exposure to nanoparticles is a constantly developing field of research. The purpose of this module therefore is to introduce to the student to particle toxicology and how it can impact upon human health. Further to this, the students will undertake a specific practical component to complement that of the theoretical content of the module. To this extent, the students will undertake a week-long, 'mini-project', covering the major areas of nanoparticle toxicology. From this approach, it is intended that the student will experience the key practical approaches taken in the field.

Dr Stephen Evans

Rhif ffôn

Cyfeiriad ebost