Professor David Lamb

Professor

Biomedical Sciences

+44 (0) 1792 602178

d.c.lamb@swansea.ac.uk

Available For Postgraduate Supervision

Research Links

https://orcid.org/0000-0001-5446-2997

About

David received his BSc in Pharmacology from the University of Liverpool and PhD in Molecular Biology/Biochemistry from the University of Sheffield. David joined Swansea Medical School as a Reader in 2004 and was appointed to a personal Chair in 2005. He is a Fellow of the Royal Society of Chemistry, Fellow of the Royal Society of Biology, Fellow of the linnean Society, and a Fulbright Scholar. He was awarded a DSc from Swansea University in 2011. He has worked on cytochrome P450 biodiversity for over 25 years. He has spent considererable time studying P450 genes/enzymes as a visiting Fellow to laboratories in the USA, including Vanderbilt University Medical School and Woods Hole Oceanographic Institute (WHOI), Human Health and Ocean Sciences. He has had research support (PI and/or co-I) from the BBSRC, NIH, NERC, Wellcome Trust, Leverhulme Trust, Nuffield Foundation and The Royal Society. In 2016/17 he was awarded a highly prestigious UK-US Fulbright Scholarship.

Areas Of Expertise

Biochemistry
Molecular Biology
Microbiology
Toxicology
Evolution

Career Highlights

Teaching Interests

David teaches both undergraduate and postgraduate students in the fields of biochemistry and molecular biology.

Award Highlights Collaborations

Publications

Research Highlights

Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
Padayachee, T., Lamb, D., Nelson, D., & Syed, K. (2023). Structure–Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family. Biomolecules, 13(12), 1733
https://doi.org/10.3390/biom13121733, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66029
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Irvine, S., Oklobia, O., Jones, S., Lamb, D., Kartopu, G., Lu, D., & Xiong, G. (2023). Creating metal saturated growth in MOCVD for CdTe solar cells. Journal of Crystal Growth, 607, 127124
https://doi.org/10.1016/j.jcrysgro.2023.127124, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62584
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
Brun, N., Salanga, M., Mora-Zamorano, F., Lamb, D., Goldstone, J., & Stegeman, J. (2021). Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), 23892
https://doi.org/10.1038/s41598-021-03068-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62080
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200
Aherfi, S., Belhaouari, D., Pinault, L., Baudoin, J., Decloquement, P., Abrahao, J., Colson, P., Levasseur, A., Lamb, D., Chabriere, E., Raoult, D., & Scola, B. (2022). Incomplete tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: is it still a virus?. The ISME Journal, 16(3), 695-704.
https://doi.org/10.1038/s41396-021-01117-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57909
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Lemaire, B., Kubota, A., O'Meara, C., Lamb, D., Tanguay, R., Goldstone, J., & Stegeman, J. (2016). Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders. Toxicology and Applied Pharmacology, 296, 73-84.
https://doi.org/10.1016/j.taap.2016.02.001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29840
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Hargrove, T., Wawrzak, Z., Lamb, D., Guengerich, F., & Lepesheva, G. (2015). Structure-Functional Characterization of Cytochrome P450 Sterol 14α-Demethylase (CYP51B) fromAspergillus fumigatusand Molecular Basis for the Development of Antifungal Drugs. Journal of Biological Chemistry, 290(39), 23916-23934.
https://doi.org/10.1074/jbc.M115.677310, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29844
http://www.jbc.org/content/290/39/23916.full.pdf
Morrison, A., Goldstone, J., Lamb, D., Kubota, A., Lemaire, B., & Stegeman, J. (2013). Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14α-demethylase. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(6), 1825-1836.
https://doi.org/10.1016/j.bbagen.2013.12.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31515
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118742/pdf/nihms584470.pdf
Lemaire, B., Karchner, S., Goldstone, J., Lamb, D., Drazen, J., Rees, J., Hahn, M., & Stegeman, J. (2018). Molecular adaptation to high pressure in cytochrome P450 1A and aryl hydrocarbon receptor systems of the deep-sea fish Coryphaenoides armatus. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
https://doi.org/10.1016/j.bbapap.2017.06.026, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34752
Goldstone, J., Sundaramoorthy, M., Zhao, B., Waterman, M., Stegeman, J., & Lamb, D. (2016). Genetic and structural analyses of cytochrome P450 hydroxylases in sex hormone biosynthesis: Sequential origin and subsequent coevolution. Molecular Phylogenetics and Evolution, 94, 676-687.
https://doi.org/10.1016/j.ympev.2015.09.012, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29843
Hargrove, T., Lamb, D., Wawrzak, Z., Hull, M., Kelly, S., Guengerich, F., & Lepesheva, G. (2024). Identification of Potent and Selective Inhibitors of Acanthamoeba: Structural Insights into Sterol 14α-Demethylase as a Key Drug Target. Journal of Medicinal Chemistry, 67(9), 7443-7457.
https://doi.org/10.1021/acs.jmedchem.4c00303, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa67193

All Research

Journal Articles

Hargrove, T., Lamb, D., Wawrzak, Z., Hull, M., Kelly, S., Guengerich, F., & Lepesheva, G. (2024). Identification of Potent and Selective Inhibitors of Acanthamoeba: Structural Insights into Sterol 14α-Demethylase as a Key Drug Target. Journal of Medicinal Chemistry, 67(9), 7443-7457.
https://doi.org/10.1021/acs.jmedchem.4c00303, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa67193
Song, Y., Amaya, J., Murarka, V., Mendez, H., Hogan, M., Muldoon, J., Evans, P., Ortin, Y., Kelly, S., Lamb, D., Poulos, T., & Caffrey, P. (2024). Biosynthesis of a new skyllamycin in Streptomyces nodosus: a cytochrome P450 forms an epoxide in the cinnamoyl chain. Organic and Biomolecular Chemistry, 22(14), 2835-2843.
https://doi.org/10.1039/d4ob00178h, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66030
Padayachee, T., Lamb, D., Nelson, D., & Syed, K. (2023). Structure–Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family. Biomolecules, 13(12), 1733
https://doi.org/10.3390/biom13121733, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa66029
Goldstone, J., Lamb, D., Kelly, S., Lepesheva, G., & Stegeman, J. (2023). Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s. Journal of Inorganic Biochemistry, 245, 112241
https://doi.org/10.1016/j.jinorgbio.2023.112241, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63599
Irvine, S., Oklobia, O., Jones, S., Lamb, D., Kartopu, G., Lu, D., & Xiong, G. (2023). Creating metal saturated growth in MOCVD for CdTe solar cells. Journal of Crystal Growth, 607, 127124
https://doi.org/10.1016/j.jcrysgro.2023.127124, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62584
Hargrove, T., Lamb, D., Smith, J., Wawrzak, Z., Kelly, S., & Lepesheva, G. (2022). Unravelling the role of transient redox partner complexes in P450 electron transfer mechanics. Scientific Reports, 12(1)
https://doi.org/10.1038/s41598-022-20671-0, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61474
Lamb, D., Goldstone, J., Zhao, B., Lei, L., Mullins, J., Allen, M., Kelly, S., & Stegeman, J. (2022). Characterization of a Virally Encoded Flavodoxin That Can Drive Bacterial Cytochrome P450 Monooxygenase Activity. Biomolecules, 12(8), 1107
https://doi.org/10.3390/biom12081107, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62081
Belhaouari, D., Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., Scola, B., & Aherfi, S. (2022). Metabolic arsenal of giant viruses: Host hijack or self-use?. eLife, 11
https://doi.org/10.7554/elife.78674, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa60435
Amaya, J., Lamb, D., Kelly, S., Caffrey, P., Murarka, V., & Poulos, T. (2022). Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s. Journal of Biological Chemistry, 298(4), 101746
https://doi.org/10.1016/j.jbc.2022.101746, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa59913
Aherfi, S., Belhaouari, D., Pinault, L., Baudoin, J., Decloquement, P., Abrahao, J., Colson, P., Levasseur, A., Lamb, D., Chabriere, E., Raoult, D., & Scola, B. (2022). Incomplete tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: is it still a virus?. The ISME Journal, 16(3), 695-704.
https://doi.org/10.1038/s41396-021-01117-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57909
Brun, N., Salanga, M., Mora-Zamorano, F., Lamb, D., Goldstone, J., & Stegeman, J. (2021). Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Scientific Reports, 11(1), 23892
https://doi.org/10.1038/s41598-021-03068-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa62080
Lamb, D., Hargrove, T., Zhao, B., Wawrzak, Z., Goldstone, J., Nes, W., Kelly, S., Waterman, M., Stegeman, J., & Lepesheva, G. (2021). Concerning P450 Evolution: Structural Analyses Support Bacterial Origin of Sterol 14α-Demethylases. Molecular Biology and Evolution, 38(3), 952-967.
https://doi.org/10.1093/molbev/msaa260, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55289
Lamb, D., Follmer, A., Goldstone, J., Nelson, D., Warrilow, A., Price, C., True, M., Kelly, S., Poulos, T., & Stegeman, J. (2019). On the occurrence of cytochrome P450 in viruses. Proceedings of the National Academy of Sciences, 116(25), 12343-12352.
https://doi.org/10.1073/pnas.1901080116, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa50802
Lemaire, B., Karchner, S., Goldstone, J., Lamb, D., Drazen, J., Rees, J., Hahn, M., & Stegeman, J. (2018). Molecular adaptation to high pressure in cytochrome P450 1A and aryl hydrocarbon receptor systems of the deep-sea fish Coryphaenoides armatus. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
https://doi.org/10.1016/j.bbapap.2017.06.026, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa34752
Lemaire, B., Kubota, A., O'Meara, C., Lamb, D., Tanguay, R., Goldstone, J., & Stegeman, J. (2016). Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders. Toxicology and Applied Pharmacology, 296, 73-84.
https://doi.org/10.1016/j.taap.2016.02.001, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29840
Goldstone, J., Sundaramoorthy, M., Zhao, B., Waterman, M., Stegeman, J., & Lamb, D. (2016). Genetic and structural analyses of cytochrome P450 hydroxylases in sex hormone biosynthesis: Sequential origin and subsequent coevolution. Molecular Phylogenetics and Evolution, 94, 676-687.
https://doi.org/10.1016/j.ympev.2015.09.012, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29843
Hargrove, T., Wawrzak, Z., Lamb, D., Guengerich, F., & Lepesheva, G. (2015). Structure-Functional Characterization of Cytochrome P450 Sterol 14α-Demethylase (CYP51B) fromAspergillus fumigatusand Molecular Basis for the Development of Antifungal Drugs. Journal of Biological Chemistry, 290(39), 23916-23934.
https://doi.org/10.1074/jbc.M115.677310, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa29844
http://www.jbc.org/content/290/39/23916.full.pdf
Lamb, D., Warrilow, A., Rolley, N., Parker, J., Nes, W., Smith, S., Kelly, D., & Kelly, S. (2015). Azole Antifungal Agents To Treat the Human Pathogens Acanthamoeba castellanii and Acanthamoeba polyphaga through Inhibition of Sterol 14α-Demethylase (CYP51). Antimicrobial Agents and Chemotherapy, 59(8), 4707-4713.
https://doi.org/10.1128/AAC.00476-15, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31514
http://aac.asm.org/content/59/8/4707.full.pdf+html
Morrison, A., Goldstone, J., Lamb, D., Kubota, A., Lemaire, B., & Stegeman, J. (2013). Identification, modeling and ligand affinity of early deuterostome CYP51s, and functional characterization of recombinant zebrafish sterol 14α-demethylase. Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(6), 1825-1836.
https://doi.org/10.1016/j.bbagen.2013.12.009, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa31515
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118742/pdf/nihms584470.pdf
Lamb, D., Skaug, T., Song, H., Jackson, C., Podust, L., Waterman, M., Kell, D., Kelly, D., & Kelly, S. (2002). The Cytochrome P450 Complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277(27), 24000-24005.
https://doi.org/10.1074/jbc.m111109200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1334
http://dx.doi.org/10.1074/jbc.m111109200

Supervision

Azole Antifungal Resistance in Plant Pathogenic Fungi (current)

PhD

Other supervisor: Prof Steven Kelly
Microbial Cytochrome P450 and Azole Antifungal Resistance (current)

PhD

Other supervisor: Dr Claire Price

Taught Modules

PM-251 Microbial Molecular Genetics

The control systems governing gene expression in prokaryotic cells are explored and the molecular basis for gene regulation introduced. Practical skills in obtaining and analysing data on a bacterial system are developed. The module then assesses the structure of the bacterial genome and how genetic analysis can be used to investigate structure and function of individual components of that genome. Specific examples explored in depth are the genetics of recombination and DNA repair. The technologies used for functional genomic analysis of fully sequenced bacteria are described with reference to analysis of human pathogens. Finally, the module includes examples of synthetic biology applied to bacteria.
PM-344 Capstone Project

The aim of this module is to provide a capstone experience to students¿ learning, through participating in their own enquiry-based research project, with guidance from an academic supervisor. The project may be laboratory or non-laboratory based, but it will always involve a research question that is drawn from the literature, and focused on a topic relevant to the life sciences. It will ask a research question and involve the critical analysis of research findings. Students will refine their oral and written communication skills to a graduate level through an oral presentation and dissertation on their research findings and conclusions.
PMGM12 Omics Techniques and their Application to Genomic Medicine

This module explores the state of the art genomics techniques used for DNA sequencing (e.g. targeted approaches, whole exome and whole genome sequencing) and RNA sequencing. This module will include introductory bioinformatics approaches required for the analysis of genomic data, which will provide a solid foundation for the Bioinformatics module. The module will also cover the use of array based methodologies and RNA sequencing in estimating levels of protein expression, micro RNAs and gene manipulation technologies. A comprehensive introduction to metabolomics and proteomics, which are important for the functional interpretation of genomic data and discovery of disease biomarkers will also be included.
PMGM15 Application of Genomics in Infectious Disease

In this module the student will learn about the genomic structure of infectious agents, implication of acquisition or loss of nucleotides, genes and plasmids on pathogenicity, sensitivity of a pathogen to drug treatment and outbreak control.
PMGM17 Post Graduate Taught Masters Dissertation

The module is for students to build on their previous knowledge, skills and experience by undertaking a laboratory based or suitable non-laboratory based project on a specific subject related to genomics.
PMGM24 Application of Genomics in Infectious Disease

In this module the student will learn about the genomic structure of infectious agents, implication of acquisition or loss of nucleotides, genes and plasmids on pathogenicity, sensitivity of a pathogen to drug treatment and outbreak control. Students will learn about infection as a cause of national and global morbidity and mortality, transmission of human infections: person to person, food and water-borne, sexually transmitted, vector-borne, prokaryotes, their¿genome, replication and population¿genetics, genomic characterisation of viruses:¿DNA¿and¿RNA¿genomes, single-stranded, double stranded, segmented, genomic comparisons of microbial strains in the context of outbreaks and transmissions in hospitals and the community, laboratory diagnosis, including genomic techniques and technologies, and appropriate sample type, analysis and interpretation of genomic data, and the role of bioinformatics, anti-infective drug action, mutation¿rate and drug resistance, genomic evidence of individual susceptibility to specific infection and the role of genomics in: infectious disease diagnosis, drug selection, resistance, monitoring and epidemic control. The students will also learn how to perform aspects of in-silico analyses on microbial gene sequences, in a workshop setting.
PMRM04 Dissertation (Research)

This module builds on the knowledge and skills developed in teaching components in part one of their relevant programme. Students will work independently in order to critically explore and add to the evidence base for a topic of relevance to their area of study.

Professor David Lamb

Telephone number

Email address

Research Links

About

Areas Of Expertise

Career Highlights

Publications

Research Highlights

All Research

Journal Articles

Supervision

Azole Antifungal Resistance in Plant Pathogenic Fungi (current)

Microbial Cytochrome P450 and Azole Antifungal Resistance (current)

Taught Modules

PM-251 Microbial Molecular Genetics

PM-344 Capstone Project

PMGM12 Omics Techniques and their Application to Genomic Medicine

PMGM15 Application of Genomics in Infectious Disease

PMGM17 Post Graduate Taught Masters Dissertation

PMGM24 Application of Genomics in Infectious Disease

PMRM04 Dissertation (Research)