Dr Paula Row

Senior Lecturer

Biomedical Sciences

+44 (0) 1792 602789

p.e.row@swansea.ac.uk

Academic Office - 104
First Floor
Institute of Life Science 1
Singleton Campus

Available For Postgraduate Supervision

About

Paula has been a member of the Biochemistry team since 2007. Her current research focus is on the antibacterial activity of essential oils and herbal medicines and their potential for treating irritable bowel syndrome, now known to involve changes in gut bacteria. She is a member of the supervisory team for an MD student researching the use of faecal transplantation for treating ulcerative colitis. Paula’s first degree was BSc Biochemistry with Extramural Year from King’s College London (1st Class Hons) and her PhD and postdoctoral research was all in Membrane Trafficking (how proteins are delivered to the correct place in cells). Her PhD was in chloroplast protein import from the Department of Plant Sciences, University of Cambridge. Paula studied protein delivery to lysosomes at the Cambridge Institute of Medical Research for her first postdoc, and for her second she investigated the role of ubiquitin in the signalling and down-regulation of growth factor receptors at the Physiological Laboratory, University of Liverpool. Paula delivers, or teaches on, a number of core biochemistry modules about hormonal regulation of biochemical pathways, clinical biochemistry, bioenergetics, membrane trafficking and biomolecular techniques, and was awarded a Teaching Contribution Award in 2008. She is the Local Ambassador for the Biochemical Society and has been a reviewer for BBSRC and Biomed Central. She is Academic Disability Coordinator for Genetics and Biochemistry.

Areas Of Expertise

Biochemistry
Membrane Trafficking, particularly of receptors
Ubiquitin
Molecular Cell Biology
Molecular and cellular basis of disease and therapeutics
Antibacterial activity

Career Highlights

Award Highlights

June 2008. Swansea University Teaching Contribution Award.
October 1991. Frank Smart Studentship, Department of Plant Sciences, University of Cambridge
June 1991. William Robson Prize in Biochemistry, King’s College, London.

Publications

Research Highlights

Row, P., Jannoo, R., Xia, Z., Row, P., & Kanamarlapudi, V. (2023). Targeting of the Interleukin-13 Receptor (IL-13R)α2 Expressing Prostate Cancer by a Novel Hybrid Lytic Peptide. Biomolecules, 13(2), 356
https://doi.org/10.3390/biom13020356, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63097
http://dx.doi.org/10.3390/biom13020356
Wilkinson, T., Jenkins, G., Davies, A., Row, P., Hitchings, M., & Islam, S. (2018). Protocol for faecal microbiota transplantation in ulcerative colitis (FMTUC): a randomised feasibility study. BMJ Open
https://doi.org/10.1136/bmjopen-2018-021987, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45409
Thompson, A., Meah, D., Ahmed, N., Conniff-Jenkins, R., Chileshe, E., Phillips, C., Claypole, T., Forman, D., Row, P., Claypole, T., Phillips, C., & Forman, D. (2013). Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome. BMC Complementary and Alternative Medicine, 13(1), 338
https://doi.org/10.1186/1472-6882-13-338, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18384
http://www.biomedcentral.com/content/pdf/1472-6882-13-338.pdf
Bond, A., Row, P., Dudley, E., Dudley, E., & Row, P. (2011). Post-translation modification of proteins; methodologies and applications in plant sciences. Phytochemistry, 72(10), 975-996.
https://doi.org/10.1016/j.phytochem.2011.01.029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10603
Row, P., Liu, H., Hayes, S., Welchman, R., Charalabous, P., Hofmann, K., Clague, M., Sanderson, C., Urbe, S., & Row, P. (2007). The MIT Domain of UBPY Constitutes a CHMP Binding and Endosomal Localization Signal Required for Efficient Epidermal Growth Factor Receptor Degradation. Journal of Biological Chemistry, 282(42), 30929-30937.
https://doi.org/10.1074/jbc.M704009200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1511
McCullough, J., Urbé, S., Row, P., Prior, I., Welchman, R., Clague, M., & Row, P. (2006). Control of growth factor receptor dynamics by reversible ubiquitination. Biochemical Society Transactions, 34(5), 754
https://doi.org/10.1042/BST0340754, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18385
Row, P., Prior, I., McCullough, J., Clague, M., Urbé, S., & Row, P. (2006). The Ubiquitin Isopeptidase UBPY Regulates Endosomal Ubiquitin Dynamics and Is Essential for Receptor Down-regulation. Journal of Biological Chemistry, 281(18), 12618-12624.
https://doi.org/10.1074/jbc.M512615200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1512
McCullough, J., Row, P., Lorenzo, Ó., Doherty, M., Beynon, R., Clague, M., & Urbé, S. (2006). Activation of the Endosome-Associated Ubiquitin Isopeptidase AMSH by STAM, a Component of the Multivesicular Body-Sorting Machinery. Current Biology, 16(2), 160-165.
https://doi.org/10.1016/j.cub.2005.11.073, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18382
http://ac.els-cdn.com/S0960982205015241/1-s2.0-S0960982205015241-main.pdf?_tid=48af539c-38ef-11e4-949d-00000aab0f6c&acdnat=1410356289_96c0ae8c70550570797a00a792ca4c8b
Row, P., Clague, M., & Urbé, S. (2005). Growth factors induce differential phosphorylation profiles of the Hrs–STAM complex: a common node in signalling networks with signal-specific properties. Biochemical Journal, 389(3), 629
https://doi.org/10.1042/BJ20050067, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1513
Cook, N., Row, P., & Davidson, H. (2004). Lysosome Associated Membrane Protein 1 (Lamp1) Traffics Directly from the TGN to Early Endosomes. Traffic, 5(9), 685-699.
https://doi.org/10.1111/j.1600-0854.2004.00212.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18381
http://onlinelibrary.wiley.com/store/10.1111/j.1600-0854.2004.00212.x/asset/j.1600-0854.2004.00212.x.pdf?v=1&t=hzwpbpou&s=b8b7d06384e9820167203022a49642432b43aa96
Urbe, S., Sachse, M., Row, P., Preisinger, C., Barr, F., Strous, G., Klumperman, J., & Clague, M. (2003). The UIM domain of Hrs couples receptor sorting to vesicle formation. Journal of Cell Science, 116(20), 4169-4179.
https://doi.org/10.1242/jcs.00723, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18377
http://jcs.biologists.org/content/116/20/4169.full.pdf
Row, P. & Gray, J. (2001). The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates. Journal of Experimental Botany, 52(354), 57-66.
https://doi.org/10.1093/jexbot/52.354.57, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18376
http://jxb.oxfordjournals.org/content/52/354/57.full.pdf
Row, P. & Gray, J. (2001). Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts. Journal of Experimental Botany, 52(354), 47-56.
https://doi.org/10.1093/jexbot/52.354.47, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18375
http://jxb.oxfordjournals.org/content/52/354/47.full.pdf
Row, P., REAVES, B., DOMIN, J., LUZIO, J., & DAVIDSON, H. (2001). Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation. Biochemical Journal, 353(3), 655-661.
https://doi.org/10.1042/bj3530655, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18374
Reaves, B., Row, P., Bright, N., Luzio, J., & Davidson, H. (2000). Loss of cation-independent mannose 6-phosphate receptor expression promotes the accumulation of lysobisphosphatidic acid in multilamellar bodies. Journal of Cell Science, 113, 4099-4108.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18373
http://jcs.biologists.org/content/113/22/4099.full.pdf
Gray, J. & Row, P. (1995). Protein translocation across chloroplast envelope membranes. Trends in Cell Biology, 5(6), 243-247.
https://doi.org/10.1016/S0962-8924(00)89018-2, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18372
http://www.sciencedirect.com/science/article/pii/S0962892400890182

All Research

Journal Articles

Row, P., Jannoo, R., Xia, Z., Row, P., & Kanamarlapudi, V. (2023). Targeting of the Interleukin-13 Receptor (IL-13R)α2 Expressing Prostate Cancer by a Novel Hybrid Lytic Peptide. Biomolecules, 13(2), 356
https://doi.org/10.3390/biom13020356, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa63097
http://dx.doi.org/10.3390/biom13020356
Wilkinson, T., Jenkins, G., Davies, A., Row, P., Hitchings, M., & Islam, S. (2018). Protocol for faecal microbiota transplantation in ulcerative colitis (FMTUC): a randomised feasibility study. BMJ Open
https://doi.org/10.1136/bmjopen-2018-021987, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa45409
Thompson, A., Meah, D., Ahmed, N., Conniff-Jenkins, R., Chileshe, E., Phillips, C., Claypole, T., Forman, D., Row, P., Claypole, T., Phillips, C., & Forman, D. (2013). Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome. BMC Complementary and Alternative Medicine, 13(1), 338
https://doi.org/10.1186/1472-6882-13-338, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18384
http://www.biomedcentral.com/content/pdf/1472-6882-13-338.pdf
Bond, A., Row, P., Dudley, E., Dudley, E., & Row, P. (2011). Post-translation modification of proteins; methodologies and applications in plant sciences. Phytochemistry, 72(10), 975-996.
https://doi.org/10.1016/j.phytochem.2011.01.029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa10603
Row, P., Liu, H., Hayes, S., Welchman, R., Charalabous, P., Hofmann, K., Clague, M., Sanderson, C., Urbe, S., & Row, P. (2007). The MIT Domain of UBPY Constitutes a CHMP Binding and Endosomal Localization Signal Required for Efficient Epidermal Growth Factor Receptor Degradation. Journal of Biological Chemistry, 282(42), 30929-30937.
https://doi.org/10.1074/jbc.M704009200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1511
McCullough, J., Urbé, S., Row, P., Prior, I., Welchman, R., Clague, M., & Row, P. (2006). Control of growth factor receptor dynamics by reversible ubiquitination. Biochemical Society Transactions, 34(5), 754
https://doi.org/10.1042/BST0340754, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18385
Row, P., Prior, I., McCullough, J., Clague, M., Urbé, S., & Row, P. (2006). The Ubiquitin Isopeptidase UBPY Regulates Endosomal Ubiquitin Dynamics and Is Essential for Receptor Down-regulation. Journal of Biological Chemistry, 281(18), 12618-12624.
https://doi.org/10.1074/jbc.M512615200, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1512
McCullough, J., Row, P., Lorenzo, Ó., Doherty, M., Beynon, R., Clague, M., & Urbé, S. (2006). Activation of the Endosome-Associated Ubiquitin Isopeptidase AMSH by STAM, a Component of the Multivesicular Body-Sorting Machinery. Current Biology, 16(2), 160-165.
https://doi.org/10.1016/j.cub.2005.11.073, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18382
http://ac.els-cdn.com/S0960982205015241/1-s2.0-S0960982205015241-main.pdf?_tid=48af539c-38ef-11e4-949d-00000aab0f6c&acdnat=1410356289_96c0ae8c70550570797a00a792ca4c8b
Row, P., Clague, M., & Urbé, S. (2005). Growth factors induce differential phosphorylation profiles of the Hrs–STAM complex: a common node in signalling networks with signal-specific properties. Biochemical Journal, 389(3), 629
https://doi.org/10.1042/BJ20050067, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa1513
Cook, N., Row, P., & Davidson, H. (2004). Lysosome Associated Membrane Protein 1 (Lamp1) Traffics Directly from the TGN to Early Endosomes. Traffic, 5(9), 685-699.
https://doi.org/10.1111/j.1600-0854.2004.00212.x, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18381
http://onlinelibrary.wiley.com/store/10.1111/j.1600-0854.2004.00212.x/asset/j.1600-0854.2004.00212.x.pdf?v=1&t=hzwpbpou&s=b8b7d06384e9820167203022a49642432b43aa96
Urbe, S., Sachse, M., Row, P., Preisinger, C., Barr, F., Strous, G., Klumperman, J., & Clague, M. (2003). The UIM domain of Hrs couples receptor sorting to vesicle formation. Journal of Cell Science, 116(20), 4169-4179.
https://doi.org/10.1242/jcs.00723, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18377
http://jcs.biologists.org/content/116/20/4169.full.pdf
Row, P. & Gray, J. (2001). The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates. Journal of Experimental Botany, 52(354), 57-66.
https://doi.org/10.1093/jexbot/52.354.57, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18376
http://jxb.oxfordjournals.org/content/52/354/57.full.pdf
Row, P. & Gray, J. (2001). Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts. Journal of Experimental Botany, 52(354), 47-56.
https://doi.org/10.1093/jexbot/52.354.47, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18375
http://jxb.oxfordjournals.org/content/52/354/47.full.pdf
Row, P., REAVES, B., DOMIN, J., LUZIO, J., & DAVIDSON, H. (2001). Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation. Biochemical Journal, 353(3), 655-661.
https://doi.org/10.1042/bj3530655, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18374
Reaves, B., Row, P., Bright, N., Luzio, J., & Davidson, H. (2000). Loss of cation-independent mannose 6-phosphate receptor expression promotes the accumulation of lysobisphosphatidic acid in multilamellar bodies. Journal of Cell Science, 113, 4099-4108.
SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18373
http://jcs.biologists.org/content/113/22/4099.full.pdf
Gray, J. & Row, P. (1995). Protein translocation across chloroplast envelope membranes. Trends in Cell Biology, 5(6), 243-247.
https://doi.org/10.1016/S0962-8924(00)89018-2, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa18372
http://www.sciencedirect.com/science/article/pii/S0962892400890182

Supervision

The microbiota in ulcerative colitis: a feasability study for a clinical trial of faecal microbiota transplant in selected cases (awarded 2022)

MD

Other supervisor: Prof Angharad Davies

Taught Modules

PM-127 Energy and Metabolism: The Reactions of Life

This module provides an introduction to bioenergetics and the chemical structure of carbohydrates as well as a discussion of the central metabolic pathways by which energy from food is used to fuel the synthesis of the energy currency molecule ATP (with particular attention to glycolysis, the citric acid cycle and oxidative phosphorylation). The concept of tissue and organ specificity is introduced and a brief introduction to hormonal regulation by insulin and glucagon.
PM-130 Fundamental Research Skills

This module is designed to develop the skills required for students of biochemistry and genetics degree programmes. Students meet with their tutors and will be given a series of assignments designed to develop skills in key areas such as essay writing, presentations and general numeracy.
PM-243 Clinical Biochemistry & Physiology

The module begins with a discussion of selected metabolic disorders of carbohydrate metabolism and their pathological consequences with particular emphasis on diabetes mellitus. The molecular mechanisms underlying polycystic ovarian syndrome are then considered. Next, there will be a discussion of the analytical tests used by the NHS to diagnose and monitor diseases such as diabetes (for instance the glycated haemoglobin or Hba1c test) how the test(s) can be affected by haemoglobinopathies and the length of time that has elapsed since someone has taken their medication. Finally the module reviews mechanisms of protein transport and their relevance to storage diseases.
PM-245 Metabolic Regulation: Enzymes & Signal Transduction

This module reviews the basic elements of enzyme kinetics, including their mechanism and inhibition. The control of enzyme function by allosteric mechanisms is considered together with an appreciation of the significance of sigmoid kinetics. An overview of coenzymes and (metal) co-factors in enzyme function is given together with a description of the role played by vitamins in co-enzyme production.
PM-277 Techniques in Molecular Biology

The module will provide a sound theoretical basis to a comprehensive range of biomolecular techniques currently employed in molecular genetics and biochemistry. Particular emphasis is placed on DNA recombinant technology, next generation sequencing, transcriptomics, proteomics and mass spectrometry.
PM-277C Technegau Bioleg Foleciwlaidd

Bydd y modiwl yn darparu sylfaen ddamcaniaethol gadarn ar gyfer ystod gynhwysfawr o dechnegau biofoleciwlaidd a ddefnyddir ar hyn o bryd mewn geneteg foleciwlaidd a biocemeg. Rhoddir pwyslais arbennig ar dechnoleg ail-gyfuno DNA, dilyniannu¿r genhedlaeth nesaf, trawsgrifiadomeg, proteinomeg a sbectrometreg màs.
PM-280 Skills for Researchers

The modern scientist graduate is expected to have gained extensive laboratory-based experience and this is certainly essential for any student intent on pursuing a research-based career. Skills for Researchers provides students with the opportunity to acquire the core skills to be able to conduct, analyse, interpret and present scientific experiments. A proportion of the module will be spent in the laboratory gaining hands-on experience. Skills acquired in this module will form the basis for future experimental work, including the final year project. Students will have the opportunity to provide feedback to peers and engage with a variety of online learning materials.
PM-319 Membranes and Energy Transduction

An appreciation of the molecular architecture of energy-conserving membranes is provided together with an understanding of the molecular mechanisms involved in energy transduction. The course also provides an understanding of the structure and properties of membranes, the lipids contained within them and the techniques used to study them.
PM-331 Membrane Trafficking

The module provides an understanding of the subcellular organelles, the regulation of their biosynthesis and how impairment of this process leads to disease.
PM-344 Capstone Project

The aim of this module is to provide a capstone experience to students¿ learning, through participating in their own enquiry-based research project, with guidance from an academic supervisor. The project may be laboratory or non-laboratory based, but it will always involve a research question that is drawn from the literature, and focused on a topic relevant to the life sciences. It will ask a research question and involve the critical analysis of research findings. Students will refine their oral and written communication skills to a graduate level through an oral presentation and dissertation on their research findings and conclusions.
PMRM04 Dissertation (Research)

This module builds on the knowledge and skills developed in teaching components in part one of their relevant programme. Students will work independently in order to critically explore and add to the evidence base for a topic of relevance to their area of study.

Career History

Lecturer / Senior Lecturer in Biochemistry , Swansea University

2007 - Present
Postdoctoral Research Associate , The Physiological Laboratory, University of Liverpool

October 2002 - September 2006
Postdoctoral Research Associate , Department of Clinical Biochemistry, University of Cambridge and Cambridge Institute for Medical Research.

August 1996 - July 2002
PhD Student , Department of Plant Sciences, University of Cambridge and St John’s College, Cambridge

October 1991 - July 1996
Student reading for a BSc in Biochemistry with Extramural year , King’s College, London

October 1987 - July 1991
Extramural year student , Plant Breeding Institute, Trumpington and the John Innes Institute, Norwich.

1989 - 1990

Affiliated Positions

Disability Coordinator, Swansea University

2013 - Present
Disability Coordinator for SUMS research students, Swansea University

2013 - 2018
Swansea Academy of Learning and Teaching (SALT) Lead for SUMS, Swansea University

2013 - Present
Academic Lead (SAILS), Swansea University

2017 - Present
Member- Swansea University Medical School Research Committee, Swansea University

2016 - Present
Member (Teaching board), Swansea University

2007 - Present
Member (SUMS Learning and Teaching Committee), Swansea University

2013 - Present

Dr Paula Row

Telephone number

Email address

About

Areas Of Expertise

Career Highlights

Publications

Research Highlights

All Research

Journal Articles

Supervision

The microbiota in ulcerative colitis: a feasability study for a clinical trial of faecal microbiota transplant in selected cases (awarded 2022)

Taught Modules

PM-127 Energy and Metabolism: The Reactions of Life

PM-130 Fundamental Research Skills

PM-243 Clinical Biochemistry & Physiology

PM-245 Metabolic Regulation: Enzymes & Signal Transduction

PM-277 Techniques in Molecular Biology

PM-277C Technegau Bioleg Foleciwlaidd

PM-280 Skills for Researchers

PM-319 Membranes and Energy Transduction

PM-331 Membrane Trafficking

PM-344 Capstone Project

PMRM04 Dissertation (Research)

Career History

Lecturer / Senior Lecturer in Biochemistry , Swansea University

Postdoctoral Research Associate , The Physiological Laboratory, University of Liverpool

Postdoctoral Research Associate , Department of Clinical Biochemistry, University of Cambridge and Cambridge Institute for Medical Research.

PhD Student , Department of Plant Sciences, University of Cambridge and St John’s College, Cambridge

Student reading for a BSc in Biochemistry with Extramural year , King’s College, London

Extramural year student , Plant Breeding Institute, Trumpington and the John Innes Institute, Norwich.

Affiliated Positions

Disability Coordinator, Swansea University

Disability Coordinator for SUMS research students, Swansea University

Swansea Academy of Learning and Teaching (SALT) Lead for SUMS, Swansea University

Academic Lead (SAILS), Swansea University

Member- Swansea University Medical School Research Committee, Swansea University

Member (Teaching board), Swansea University

Member (SUMS Learning and Teaching Committee), Swansea University