Institute of Life Science 1 internal Atrium view up.jpg

Dr Ume-kulsoom Shah

Technician (Life Science Research)

Faculty of Medicine Health and Life Science

u.shah@swansea.ac.uk

Academic Office - 429
Fourth Floor
Institute of Life Science 1
Singleton Campus

Research Links

https://orcid.org/0000-0002-0594-580X

ResearchGate

About

Ume-Kulsoom works as the research technician on the 4th floor in Invitro Toxicology group in the medical school. She maintains the laboratory to research standards. She supervised BSc project students in DNA damage projects. She trains and assists new members of staff and visitors. She is a passionate researcher trying to add knowledge regarding factors influencing carcinogenicity and genotoxicity of different chemicals present in environment and used in food and items of daily use.

Areas Of Expertise

Genotoxicity assay
Mutation Assay
Confocal microscopy
Quantitative assays like PCR and qPCR
Qualitative assay e.g. Western blot
Confocal Imaging
Data analysis

Career Highlights

Teaching Interests

Provide support in practical classes and shadowed Kate Chapman to learn teaching skills

Research

Publications

Research Highlights

Seager, A., Shah, U., Brusehafer, K., Wills, J., Manshian, B., Chapman, K., Thomas, A., Scott, A., Doherty, A., Doak, S., Johnson, G., Jenkins, G., Jenkins, G., Doak, S., Seager, A., Johnson, G., Bruesehafer, K., Shah, U., & Chapman, K. (2014). Recommendations, evaluation and validation of a semi-automated, fluorescent-based scoring protocol for micronucleus testing in human cells. Mutagenesis, 29(3), 155-164.
https://doi.org/10.1093/mutage/geu008, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa17804
Shah, U., Mallia, J., Singh, N., Chapman, K., Doak, S., & Jenkins, G. (2018). A three-dimensional in vitro HepG2 cells liver spheroid model for genotoxicity studies. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 825, 51-58.
https://doi.org/10.1016/j.mrgentox.2017.12.005, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37674
Llewellyn, S., Conway, G., Shah, U., Evans, S., Jenkins, G., Clift, M., & Doak, S. (2020). Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. Journal of Visualized Experiments(160)
https://doi.org/10.3791/61141, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54416
Conway, G., Shah, U., Llewellyn, S., Cervena, T., Evans, S., Al-Ali, A., Jenkins, G., Clift, M., & Doak, S. (2020). Adaptation of the in vitro micronucleus assay for genotoxicity testing using 3D liver models supporting longer-term exposure durations. Mutagenesis, 35(4), 319-329.
https://doi.org/10.1093/mutage/geaa018, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54991
Chapman, K., Wilde, E., Chapman, F., Verma, J., Shah, U., Stannard, L., Seager, A., Tonkin, J., Brown, R., Doherty, A., Johnson, G., Doak, S., & Jenkins, G. (2021). Multiple-endpoint in vitro carcinogenicity test in human cell line TK6 distinguishes carcinogens from non-carcinogens and highlights mechanisms of action. Archives of Toxicology, 95(1), 321-336.
https://doi.org/10.1007/s00204-020-02902-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55186
Shah, U., Verma, J., Chapman, K., Wilde, E., Tonkin, J., Brown, R., Johnson, G., Doak, S., & Jenkins, G. (2020). Detection of urethane-induced genotoxicity in vitro using metabolically competent human 2D and 3D spheroid culture models. Mutagenesis, 35(6), 445-452.
https://doi.org/10.1093/mutage/geaa029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55770
Dural, E., Shah, U., Pritchard, D., Chapman, K., Doak, S., Jenkins, G., & Jenkins, G. (2020). The effect of chronic dosing and p53 status on the genotoxicity of pro-oxidant chemicals in vitro. Mutagenesis, 35(6), 479-489.
https://doi.org/10.1093/mutage/geaa024, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55854
Llewellyn, S., Conway, G., Zanoni, I., Jørgensen, A., Shah, U., Seleci, D., Keller, J., Kim, J., Wohlleben, W., Jensen, K., Costa, A., Jenkins, G., Clift, M., & Doak, S. (2021). Understanding the impact of more realistic low-dose, prolonged engineered nanomaterial exposure on genotoxicity using 3D models of the human liver. Journal of Nanobiotechnology, 19(1)
https://doi.org/10.1186/s12951-021-00938-w, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57237
Llewellyn, S., Kermanizadeh, A., Ude, V., Jacobsen, N., Conway, G., Shah, U., Niemeijer, M., Moné, M., Water, B., Roy, S., Moritz, W., Stone, V., Jenkins, G., & Doak, S. (2022). Assessing the transferability and reproducibility of 3D in vitro liver models from primary human multi-cellular microtissues to cell-line based HepG2 spheroids. Toxicology in Vitro, 85, 105473
https://doi.org/10.1016/j.tiv.2022.105473, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61191
Chapman, K., Shah, U., Fletcher, J., Johnson, G., Doak, S., & Jenkins, G. (2024). An integrated in vitro carcinogenicity test that distinguishes between genotoxic carcinogens, non-genotoxic carcinogens, and non-carcinogens. Mutagenesis, 39(2), 69-77.
https://doi.org/10.1093/mutage/geae004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65653

All Research

Journal Articles

Chapman, K., Shah, U., Fletcher, J., Johnson, G., Doak, S., & Jenkins, G. (2024). An integrated in vitro carcinogenicity test that distinguishes between genotoxic carcinogens, non-genotoxic carcinogens, and non-carcinogens. Mutagenesis, 39(2), 69-77.
https://doi.org/10.1093/mutage/geae004, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa65653
Llewellyn, S., Kermanizadeh, A., Ude, V., Jacobsen, N., Conway, G., Shah, U., Niemeijer, M., Moné, M., Water, B., Roy, S., Moritz, W., Stone, V., Jenkins, G., & Doak, S. (2022). Assessing the transferability and reproducibility of 3D in vitro liver models from primary human multi-cellular microtissues to cell-line based HepG2 spheroids. Toxicology in Vitro, 85, 105473
https://doi.org/10.1016/j.tiv.2022.105473, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa61191
Llewellyn, S., Conway, G., Zanoni, I., Jørgensen, A., Shah, U., Seleci, D., Keller, J., Kim, J., Wohlleben, W., Jensen, K., Costa, A., Jenkins, G., Clift, M., & Doak, S. (2021). Understanding the impact of more realistic low-dose, prolonged engineered nanomaterial exposure on genotoxicity using 3D models of the human liver. Journal of Nanobiotechnology, 19(1)
https://doi.org/10.1186/s12951-021-00938-w, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa57237
Chapman, K., Wilde, E., Chapman, F., Verma, J., Shah, U., Stannard, L., Seager, A., Tonkin, J., Brown, R., Doherty, A., Johnson, G., Doak, S., & Jenkins, G. (2021). Multiple-endpoint in vitro carcinogenicity test in human cell line TK6 distinguishes carcinogens from non-carcinogens and highlights mechanisms of action. Archives of Toxicology, 95(1), 321-336.
https://doi.org/10.1007/s00204-020-02902-3, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55186
Shah, U., Verma, J., Chapman, K., Wilde, E., Tonkin, J., Brown, R., Johnson, G., Doak, S., & Jenkins, G. (2020). Detection of urethane-induced genotoxicity in vitro using metabolically competent human 2D and 3D spheroid culture models. Mutagenesis, 35(6), 445-452.
https://doi.org/10.1093/mutage/geaa029, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55770
Dural, E., Shah, U., Pritchard, D., Chapman, K., Doak, S., Jenkins, G., & Jenkins, G. (2020). The effect of chronic dosing and p53 status on the genotoxicity of pro-oxidant chemicals in vitro. Mutagenesis, 35(6), 479-489.
https://doi.org/10.1093/mutage/geaa024, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa55854
Conway, G., Shah, U., Llewellyn, S., Cervena, T., Evans, S., Al-Ali, A., Jenkins, G., Clift, M., & Doak, S. (2020). Adaptation of the in vitro micronucleus assay for genotoxicity testing using 3D liver models supporting longer-term exposure durations. Mutagenesis, 35(4), 319-329.
https://doi.org/10.1093/mutage/geaa018, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54991
Llewellyn, S., Conway, G., Shah, U., Evans, S., Jenkins, G., Clift, M., & Doak, S. (2020). Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure. Journal of Visualized Experiments(160)
https://doi.org/10.3791/61141, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa54416
Shah, U., Mallia, J., Singh, N., Chapman, K., Doak, S., & Jenkins, G. (2018). A three-dimensional in vitro HepG2 cells liver spheroid model for genotoxicity studies. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 825, 51-58.
https://doi.org/10.1016/j.mrgentox.2017.12.005, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa37674
Seager, A., Shah, U., Brusehafer, K., Wills, J., Manshian, B., Chapman, K., Thomas, A., Scott, A., Doherty, A., Doak, S., Johnson, G., Jenkins, G., Jenkins, G., Doak, S., Seager, A., Johnson, G., Bruesehafer, K., Shah, U., & Chapman, K. (2014). Recommendations, evaluation and validation of a semi-automated, fluorescent-based scoring protocol for micronucleus testing in human cells. Mutagenesis, 29(3), 155-164.
https://doi.org/10.1093/mutage/geu008, SU Repository: https://cronfa.swan.ac.uk/Record/cronfa17804